AB196396

重组人PRDM14蛋白

Name: 重组人PRDM14蛋白 (ab196396)| Abcam中文官网
Brand: Abcam Limited
SKU: AB196396
Price: 14181 CNY
Availability: InStock

Recombinant Human PRDM14 protein

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(1 Publication)

Recombinant Human PRDM14 protein is a Human Fragment protein, in the 2 to 412 aa range, expressed in Escherichia coli, with >70%, suitable for SDS-PAGE.

查看别名

PR domain zinc finger protein 14, PR domain-containing protein 14, PRDM14

1 Images

SDS-PAGE - Recombinant Human PRDM14 protein (AB196396)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PRDM14 protein (AB196396)

4-20% SDS-PAGE with Coomassie staining : 2 μg ab196396.

关键信息

纯度

>70% SDS-PAGE

表达系统

Escherichia coli

应用

SDS-PAGE

applications

生物活性

访问

Q9GZV8

不含动物源

不含载体蛋白

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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序列信息

[{"sequence":"MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQ^GPLGSALPRPSEAVPQDKVCYPPESSPQNLAAYYTPFPSYGHYRNSLATVEEDFQPFRQLEAAASAAPAMPPFPFRMAPPLLSPGLGLQREPLYDLPWYSKLPPWYPIPHVPREVPPFLSSSHEYAGASSEDLGHQIIGGDNESGPCCGPDTLIPPPPADASLLPEGLRTSQLLPCSPSKQSEDGPKPSNQEGKSPARFQFTEEDLHFVLYGVTPSLEHPASLHHAISGLLVPPDSSGSDSLPQTLDKDSLQLPEGLCLMQTVFGEVPHFGVFCSSFIAKGVRFGPFQGKVVNASEVKTYGDNSVMWEIFEDGHLSHFIDGKGGTGNWMSYVNCARFPKEQNLVAVQCQGHIFYESCKEIHQNQELLVWYGDCYEKFLDIPVSLQVTEPGKQPSGPSEESAEGYRCERCGKVFTYK","proteinLength":"Fragment","predictedMolecularWeight":"72 kDa","actualMolecularWeight":null,"aminoAcidEnd":412,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9GZV8","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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性能和储存信息

运输条件

Dry Ice

储存信息

Avoid freeze / thaw cycle

False

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PRDM14 also known as PR domain zinc finger protein 14 acts as a transcription factor with a mass of approximately 64 kDa. It contains an N-terminal PR (PRDI-BF1 and RIZ) domain and zinc-finger motifs within its structure which participate in DNA binding. The protein expresses highly in embryonic stem cells and primordial germ cells showing a strong presence in pluripotent cell states.

Biological function summary

PRDM14 plays a vital role in the maintenance of pluripotency and regulation of gene expression during early embryonic development. It forms part of a regulatory network that includes other factors like OCT4 and SOX2 promoting stem cell self-renewal and inhibiting differentiation. This protein directly influences the expression of genes related to germ cell formation.

Pathways

PRDM14 interacts with several key signaling pathways notably the stem cell pluripotency pathway and the Wnt signaling pathway. It collaborates closely with proteins such as NANOG and TET proteins modulating gene expression patterns essential for pluripotent stem cell identity. Through these pathways PRDM14 ensures proper cell fate decisions during development.

PRDM14’s dysfunctions associate with certain cancers and reproductive health issues. Aberrant expression or mutation of PRDM14 appear in germ cell tumors as its regulatory functions become disrupted. Additionally PRDM14 may link to infertility disorders due to its role in germ cell development. Alterations in its activity can disrupt interactions with proteins like PRDM1 impacting normal cell proliferation and differentiation.

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特殊说明

形式

Liquid

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常规信息

功能

Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of embryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels : the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation (By similarity). Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.

序列相似性

Belongs to the class V-like SAM-binding methyltransferase superfamily.

亚细胞定位

Nucleus

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information PR domain zinc finger protein 14

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 109:373-383 PubMed29178343

2017

PRDM14 directly interacts with heat shock proteins HSP90α and glucose-regulated protein 78.

Applications

Unspecified application

Species

Unspecified reactive species

Chiharu Moriya,Hiroaki Taniguchi,Satoru Nagatoishi,Hisayoshi Igarashi,Kouhei Tsumoto,Kohzoh Imai

View all publications

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重组人PRDM14蛋白

Recombinant Human PRDM14 protein

SDS-PAGE - Recombinant Human PRDM14 protein (AB196396)

关键信息

纯度

表达系统

标签

应用

生物活性

访问

不含动物源

不含载体蛋白

种属

存储溶液