重组人PPP2CB蛋白(ab128562)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE
描述
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产品名称
重组人PPP2CB蛋白 -
纯度
> 75 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
59 kDa including tags -
氨基酸
1 to 309 -
标签
GST tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab128562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
SDS-PAGE
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形式
Liquid -
补充说明
This product was previously labelled as PP2A beta
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
常规信息
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别名
- PP2A beta
- PP2A-beta
- PP2AB_HUMAN
see all -
功能
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. -
序列相似性
Belongs to the PPP phosphatase family. PP-1 subfamily. -
翻译后修饰
Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. Demethylated by PME1 (in vitro).
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
细胞定位
Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. - Information by UniProt
图片
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All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/20000 dilution
Lane 1 :Recombinant Human PPP2CB protein (ab128562)
Lane 2 : Recombinant human PP2A-alpha protein (ab128557)
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Observed band size: 59 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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SDS-PAGE showing ab128562 at approximately 59 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab128562 尚未被引用在任何文献中。