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AB128551

重组人PPP1CB (ab128551)

Recombinant human PPP1CB

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(1 Publication)

Recombinant human PPP1CB is a Human Full Length protein, in the 1 to 327 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, WB, Phosphatase Activity.

查看别名

Serine/threonine-protein phosphatase PP1-beta catalytic subunit, PP-1B, PPP1CD, PPP1CB

4 Images
Phosphatase Activity - Recombinant human PPP1CB (AB128551)
  • Phosphatase Activity

Unknown

Phosphatase Activity - Recombinant human PPP1CB (AB128551)

Phosphatase activity demonstrating specific activity of ab128551.

Functional Studies - Recombinant human PPP1CB (AB128551)
  • FuncS

Unknown

Functional Studies - Recombinant human PPP1CB (AB128551)

The specific activity of PPP1CB (ab128551) was determined to be 313 nmol/min/mg as per activity assay protocol

Western blot - Recombinant human PPP1CB (AB128551)
  • WB

Unknown

Western blot - Recombinant human PPP1CB (AB128551)

All lanes:

Western blot - Recombinant human PPP1CB (ab128551)

false

SDS-PAGE - Recombinant human PPP1CB (AB128551)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PPP1CB (AB128551)

SDS PAGE analysis of ab128551

关键信息

纯度

>70% Densitometry

表达系统

Baculovirus infected Sf9 cells

标签

GST tag N-Terminus

应用

Phosphatase Activity, WB, SDS-PAGE

applications

生物活性

Yes

生物学活性

The specific activity of ab128551 was determined to be 272 nmol/min/mg

访问

P62140

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.02% PMSF, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Phosphatase Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Centrifuge before aliquoting into smaller quantities. This product was previously labelled as Protein Phosphatase 1 beta, PPP1CA + 1CB
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序列信息

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"60 kDa","actualMolecularWeight":null,"aminoAcidEnd":327,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P62140","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PPP1CB also known as Protein Phosphatase 1 Catalytic Subunit Beta is a member of the catalytic subunit of Protein Phosphatase 1 (PP1). It has an approximate mass of 37 kDa. PPP1CB is expressed in various tissues throughout the human body including the heart brain and skeletal muscle. This phosphatase is primarily involved in the dephosphorylation of proteins a critical function to regulate cellular processes. As a catalytic subunit PPP1CB mediates various signaling cascades by removing phosphate groups from target proteins.
Biological function summary

PPP1CB plays key roles in cell division signal transduction and muscle contraction. It acts as part of a larger complex interacting with a variety of regulatory proteins and substrates to fine-tune cellular activities. Through dephosphorylation PPP1CB aids in the transition between different phases of cell cycles impacting cellular growth and proliferation. It is also involved in glycogen metabolism where it removes phosphate groups from glycogen phosphorylase ensuring proper energy balance in cells.

Pathways

PPP1CB participates significantly in the TGF-beta signaling and Wnt signaling pathways. It interacts with several proteins such as SMADs in the TGF-beta pathway contributing to the regulation of transcription and cellular responses to growth factors. In the Wnt signaling pathway PPP1CB deactivates key kinase members thereby modulating cell fate decisions and embryonic development. These pathways underline the phosphate turnover that PPP1CB controls impacting various cellular functions.

PPP1CB has been associated with cancer development and cardiovascular diseases. It contributes to oncogenesis when its regulatory role in cell cycle controls is disrupted leading to unchecked cell proliferation. Alterations in PPP1CB activity have also been linked to heart diseases where improper dephosphorylation affects muscle contraction and cardiac function. In cancer interactions with proteins such as PP2A alter the signaling balance while in heart disease proteins like PLN may connect to aberrant PPP1CB function emphasizing its critical role in maintaining cellular health.
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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed : 23396208). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed : 35768504, PubMed : 35831509, PubMed : 36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed : 35768504, PubMed : 35831509, PubMed : 36175670). The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c (PubMed : 35768504, PubMed : 36175670).

序列相似性

Belongs to the PPP phosphatase family. PP-1 subfamily.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed : 23396208). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed : 35768504, PubMed : 35831509, PubMed : 36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed : 35768504, PubMed : 35831509, PubMed : 36175670). The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c (PubMed : 35768504, PubMed : 36175670).
See full target information PPP1CB
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 129:1340-54 PubMed26872783

2016

PP1 initiates the dephosphorylation of MASTL, triggering mitotic exit and bistability in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Rogers,Dirk Fey,Rachael A McCloy,Benjamin L Parker,Nicholas J Mitchell,Richard J Payne,Roger J Daly,David E James,C Elizabeth Caldon,D Neil Watkins,David R Croucher,Andrew Burgess
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