重组人PPP1A/PPP1CA蛋白(ab113150)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS, Functional Studies
描述
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产品名称
重组人PPP1A/PPP1CA蛋白
参阅全部 PPP1A/PPP1CA 蛋白酶 -
生物活性
>3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37 °C, pH 7.5, using 10 mM of substrate.
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纯度
> 80 % SDS-PAGE.
ab113150 was purified using conventional chromatography techniques. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
40 kDa including tags -
氨基酸
1 to 330 -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab113150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Mass Spectrometry
Functional Studies
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质谱法
MALDI-TOF -
形式
Liquid -
补充说明
This product was previously labelled as PPP1A
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.5
Constituents: 0.002% PMSF, 0.02% DTT, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.01% Manganese chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit
- Catalytic subunit
- EC 3.1.3.16
see all -
功能
Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. -
序列相似性
Belongs to the PPP phosphatase family. PP-1 subfamily. -
细胞定位
Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles. - Information by UniProt
图片
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SDS-PAGE - Recombinant human PPP1A/PPP1CA protein (ab113150)
SDS-PAGE showing ab113150 (3 µg) under reducing conditions with coomassie blue protein stain
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab113150 尚未被引用在任何文献中。