重组人PPM1A蛋白(ab128560)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Phosphatase Activity, SDS-PAGE
描述
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产品名称
重组人PPM1A蛋白
参阅全部 PPM1A 蛋白酶 -
纯度
> 70 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
105 kDa including tags -
氨基酸
1 to 382 -
标签
GST tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab128560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Phosphatase Activity
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Protein phosphatase 2C isoform alpha
- EC 3.1.3.16
- FLJ42306
see all -
功能
Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. -
序列相似性
Belongs to the PP2C family. -
细胞定位
Nucleus. - Information by UniProt
图片
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Functional Studies - Recombinant human PPM1A protein (ab128560)The specific activity of PPM1A (ab128560) was determined to be 1380 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human PPM1A protein (ab128560)SDS PAGE analysis of ab128560 -
SDS-PAGE - Recombinant human PPM1A protein (ab128560)SDS PAGE analysis of ab128560 -
Phosphatase Activity - Recombinant human PPM1A protein (ab128560)The specific activity of ab128560 was determined to be 1200 nmol/min/mg. -
SDS-PAGE - Recombinant human PPM1A protein (ab128560)SDS-PAGE showing ab128560 at approximately 105 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab128560 尚未被引用在任何文献中。