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AB93731

重组人 PPIL2 蛋白 (His tag N-Terminus)

Recombinant human PPIL2 protein (His tag N-Terminus)

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Recombinant human PPIL2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 527 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

查看别名

RING-type E3 ubiquitin-protein ligase PPIL2, CYC4, Cyclophilin-60, Probable inactive peptidyl-prolyl cis-trans isomerase-like 2, Rotamase PPIL2, Cyclophilin-like protein Cyp-60, Cyp60, hCyP-60, PPIase, PPIL2

1 Images
SDS-PAGE - Recombinant human PPIL2 protein (His tag N-Terminus) (AB93731)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PPIL2 protein (His tag N-Terminus) (AB93731)

15% SDS Page of 3ug ab93731

关键信息

纯度

>95% SDS-PAGE

Purified using conventional chromatography techniques.

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

Specific activity is > 290 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 μmole of suc-AAPF-pNA per minute at 37°C in Tris-Hcl pH8.0 using chymotrypsin.

Activity Assay

Prepare 170 μl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. Add 10 μl of recombinant PPIL2 protein with 1 μg in assay buffer. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. Add 20 μl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) Record the increase in A405 nm for 30 minutes at 25°C.

访问

Q13356

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Proteins & Peptides

AB53870

Recombinant Cyclophilin B protein (Tag Free)

Bioactive

SDS-PAGE - Recombinant Cyclophilin B protein (Tag Free) (AB53870)

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Proteins & Peptides

AB101207

Recombinant human PPIL4 protein (His tag N-Terminus)

SDS-PAGE - Recombinant human PPIL4 protein (His tag N-Terminus) (AB101207)

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Proteins & Peptides

AB113119

Recombinant human PPIL3 protein (His tag N-Terminus)

SDS-PAGE - Recombinant human PPIL3 protein (His tag N-Terminus) (AB113119)

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Proteins & Peptides

AB78874

Recombinant human PPIH protein (Tag Free)

Bioactive

SDS-PAGE - Recombinant human PPIH protein (Tag Free) (AB78874)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGKRQHQKDKMYITCAEYTHFYGGKKPDLPQTNFRRLPFDHCSLSLQPFVYPVCTPDGIVFDLLNIVPWLKKYGTNPSNGEKLDGRSLIKLNFSKNSEGKYHCPVLFTVFTNNTHIVAVRTTGNVYAYEAVEQLNIKAKNFRDLLTDEPFSRQDIITLQDPTNLDKFNVSNFYHVKNNMKIIDPDEEKAKQDPSYYLKNTNAETRETLQELYKEFKGDEILAATMKAPEKKKVDKLNAAHYSTGKVSASFTSTAMVPETTHEAAAIDEDVLRYQFVKKKGYVRLHTNKGDLNLELHCDLTPKTCENFIRLCKKHYYDGTIFHRSIRNFVIQGGDPTGTGTGGESYWGKPFKDEFRPNLSHTGRGILSMANSGPNSNRSQFFITFRSCAYLDKKHTIFGRVVGGFDVLTAMENVESDPKTDRPKEEIRIDATTVFVDPYEEADAQIAQERKTQLKVAPETKVKSSQPQAGSQGPQTFRQGVGKYINPAATEQQRKSPQPVPLSPCPRRSPVGVLGTSAPGSSRLPDDH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":527,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q13356","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cyclophilin-like 2 (PPIL2) is a protein encoded by the PPIL2 gene. It possesses peptidyl-prolyl cis-trans isomerase activity which facilitates protein folding. PPIL2 with a molecular mass of approximately 64 kDa is involved in various cellular processes. It gets expressed in many tissues such as the brain and testis. Scientists often study it using models like human cell lines to better grasp its functions in different contexts.
Biological function summary

The protein interacts with elements of the spliceosome complex contributing to pre-mRNA processing. PPIL2 partners with other molecular chaperones to regulate protein folding and quality control within the endoplasmic reticulum. These interactions influence how proteins fold stabilize and function. The protein's role in chaperone-mediated processes highlights its impact on maintaining cellular protein homeostasis.

Pathways

PPIL2 participates in the mRNA splicing pathway where it influences gene expression. It interacts with various splicing factors such as Cyclophilin A which aids in assembling and modulating spliceosome components. Another important pathway involving PPIL2 is protein processing in the endoplasmic reticulum. PPIL2 interacts with specific proteins like U-box E3 ubiquitin ligases to manage protein degradation further controlling cellular mechanisms.

Researchers associate PPIL2 with neurodegenerative diseases and cancer. Aberrations in PPIL2's function or expression can lead to the accumulation of misfolded proteins contributing to neurodegenerative conditions like Alzheimer's disease. In cancer its interaction with various oncogenic pathways can affect tumorigenesis. PPIL2's relationship with proteins like p53 in these disorders underlines its potential role in disease progression and presents it as a target for therapeutic strategies.
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常规信息

功能

Has a ubiquitin-protein ligase activity acting as an E3 ubiquitin protein ligase or as an ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on substrates. By mediating 'Lys-48'-linked polyubiquitination of proteins could target them for proteasomal degradation (PubMed : 11435423). May also function as a chaperone, playing a role in transport to the cell membrane of BSG/Basigin for instance (PubMed : 15946952). Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity (PubMed : 20676357). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).

序列相似性

Belongs to the cyclophilin-type PPIase family. PPIL2 subfamily.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

Has a ubiquitin-protein ligase activity acting as an E3 ubiquitin protein ligase or as an ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on substrates. By mediating 'Lys-48'-linked polyubiquitination of proteins could target them for proteasomal degradation (PubMed : 11435423). May also function as a chaperone, playing a role in transport to the cell membrane of BSG/Basigin for instance (PubMed : 15946952). Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity (PubMed : 20676357). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
See full target information RING-type E3 ubiquitin-protein ligase PPIL2
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