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重组人PON2蛋白(ab53379)

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SDS-PAGE - Recombinant Human PON2 protein (ab53379)
  • Western blot - Recombinant Human PON2 protein (ab53379)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Suitable for: SDS-PAGE, WB

描述

  • 产品名称

    重组人PON2蛋白

  • 纯度

    > 95 % SDS-PAGE.

  • 表达系统

    Escherichia coli

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Human

技术指标

Our Abpromise guarantee covers the use of ab53379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Western blot

  • 形式

    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.242% Tris, 50% Glycerol

常规信息

  • 别名

    • A esterase 2
    • A-esterase 2
    • Aromatic esterase 2
    • Paraoxonase 2
    • Paraoxonase nirs
    • PON 2
    • PON2
    • PON2_HUMAN
    • Serum aryldialkylphosphatase 2
    • Serum paraoxonase/arylesterase 2
    see all

  • 功能

    Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.

  • 组织特异性

    Widely expressed with highest expression in liver, lung, placenta, testis and heart.

  • 序列相似性

    Belongs to the paraoxonase family.

  • 翻译后修饰

    The signal sequence is not cleaved.

  • 细胞定位

    Membrane.
  • Information by UniProt

图片

  • SDS-PAGE - Recombinant Human PON2 protein (ab53379)
    SDS-PAGE - Recombinant Human PON2 protein (ab53379)
    SDS Page analysis of ab53379
  • Western blot - Recombinant Human PON2 protein (ab53379)
    Western blot - Recombinant Human PON2 protein (ab53379)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab53379？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab53379 尚未被引用在任何文献中。

客户评价及客户问答

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Question

Hello: We are interested in purchasing the product with Code: ab53379, which is a Human Paraoxonase 2 Recombinant protein. But we have a few technical questions: a) Has this product been tested for activity? If yes, what is the specific activity? b) Was it prepared from inclusion bodies, or was it in the soluble fraction? c) What is the sequence (if different from the one on NCBI), and Is there a thrombin site between your His Tag and the protein? d) What would be the turn around time if we place an order for 1mg of this product? Thanks,

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Answer

Thank you for your enquiry and your interest in our products. Here are the answers to your questions regarding ab53379 - PON2 protein: a) The proteins have not been tested for biological activity but they have been successful in immunoassay applications. b) The proteins have been prepared from inclusion bodies. c) The sequence is more than likely the same as the one on NCBI. The Randox protein comes with a 4 kDa leader sequence containing a hexahistidine tag. There is an enterokinase site between the tag and the protein. d) We have this protein in stock and can ship as soon as the order is placed. I hope this helps and if I can assist further, please do not hesitate to contact me.

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