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AB307790

重组人Poliovirus Receptor/PVR蛋白(His-tag) (Active)

Recombinant Human Poliovirus Receptor/PVR Protein (His-tag) (Active)

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Recombinant Human Poliovirus Receptor/PVR Protein (His-tag) (Active) is a Human Fragment protein, in the 28 to 343 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for HPLC, SDS-PAGE, Mass Spec, FuncS.

查看别名

CD155, PVS, PVR, Poliovirus receptor, Nectin-like protein 5, NECL-5

4 Images
Functional Studies - Recombinant Human Poliovirus Receptor/PVR Protein (His-tag) (Active) (AB307790)
  • FuncS

Supplier Data

Functional Studies - Recombinant Human Poliovirus Receptor/PVR Protein (His-tag) (Active) (AB307790)

Loaded Human TIGIT, Human Fc Tag on Protein A Biosensor can bind Human PVR/CD155-His with an affinity constant of 8.21 uM as determined in BLI assay (GatorBio Prime).

Mass Spectrometry - Recombinant Human Poliovirus Receptor/PVR Protein (His-tag) (Active) (AB307790)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human Poliovirus Receptor/PVR Protein (His-tag) (Active) (AB307790)

Mass determination by ESI-TOF. Predicted MW is 35888.78 Da (+/- 10 Da by ESI-TOF). Observed MW is 35896.32 Da.

HPLC - Recombinant Human Poliovirus Receptor/PVR Protein (His-tag) (Active) (AB307790)
  • HPLC

Supplier Data

HPLC - Recombinant Human Poliovirus Receptor/PVR Protein (His-tag) (Active) (AB307790)

HPLC analysis of ab307790

SDS-PAGE - Recombinant Human Poliovirus Receptor/PVR Protein (His-tag) (Active) (AB307790)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Poliovirus Receptor/PVR Protein (His-tag) (Active) (AB307790)

SDS-PAGE analysis of ab307790

关键信息

纯度

>95% HPLC

内毒素水平

<0.005 EU/µg

表达系统

HEK 293 cells

标签

His tag C-Terminus

应用

HPLC, FuncS, Mass Spec, SDS-PAGE

applications

生物活性

Yes

生物学活性

Loaded Human TIGIT, Human Fc Tag on Protein A Biosensor can bind Human PVR/CD155-His with an affinity constant of 8.21 uM as determined in BLI assay (GatorBio Prime).

访问

P15151

不含动物源

Yes

不含载体蛋白

No

种属

Human

复溶

Reconstitute in PBS

存储溶液

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"DVVVQAPTQVPGFLGDSVTLPCYLQVPNMEVTHVSQLTWARHGESGSMAVFHQTQGPSYSESKRLEFVAARLGAELRNASLRMFGLRVEDEGNYTCLFVTFPQGSRSVDIWLRVLAKPQNTAEVQKVQLTGEPVPMARCVSTGGRPPAQITWHSDLGGMPNTSQVPGFLSGTVTVTSLWILVPSSQVDGKNVTCKVEHESFEKPQLLTVNLTVYYPPEVSISGYDNNWYLGQNEATLTCDARSNPEPTGYNWSTTMGPLPPFAVAQGAQLLIRPVDKPINTTLICNVTNALGARQAELTVQVKEGPPSEHSGISRN","proteinLength":"Fragment","predictedMolecularWeight":"35.89 kDa","actualMolecularWeight":"35.9 kDa","aminoAcidEnd":343,"aminoAcidStart":28,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P15151","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
Ambient
推荐的长期储存条件
Ambient
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Poliovirus Receptor (PVR) also known as CD155 is a cell surface glycoprotein with a molecular mass of approximately 70 kDa. This receptor is expressed on a variety of cell types including epithelial and endothelial cells immune cells and fibroblasts. Another name for PVR is Necl-5 which falls under the nectin-like molecule family. It acts as an adhesion molecule and contributes to cellular signaling and junctional complexes. The expression of PVR is widespread across multiple tissues but it exhibits stronger expression in areas such as the skin and the gastrointestinal tract.
Biological function summary

The Poliovirus Receptor plays meaningful roles in immune response modulation and cell-cell adhesion. PVR interacts with components of the immune system and forms complexes with other proteins like CD226 and TIGIT. These interactions help regulate immune cell activities especially in the context of natural killer (NK) cells and T-cells. The CD155 protein also links to migration and proliferation processes which are essential for tissue formation and repair.

Pathways

PVR is involved in the regulation of immune and signaling pathways. It fits into pathways like NK cell activation and T-cell inhibitory signaling which are important for maintaining immune tolerance and preventing autoimmunity. In these pathways PVR interacts closely with other immunoregulatory proteins including CD226 and TIGIT. The partnership of PVR with these proteins shapes the delicate balance between immune activation and suppression demonstrating a clear role in immune homeostasis.

PVR relates to conditions such as cancer and viral infection. Its overexpression or altered signaling has been observed in several cancers where it may contribute to tumor growth and immune evasion. The interaction between PVR and its related protein TIGIT can affect antitumor immune responses complicating cancer progression. Additionally as its name suggests PVR binds to the poliovirus facilitating viral entry and spread during infection. This highlights the importance of PVR not only in pathogenic interactions but also in broader immune response contexts.
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特殊说明

形式

Lyophilized

附加说明

SDS-PAGE >= 95%

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常规信息

功能

Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors : CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration.. (Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.. (Microbial infection) Acts as a receptor for Pseudorabies virus.. (Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells.

序列相似性

Belongs to the nectin family.

翻译后修饰

N-glycosylated. N-glycan at Asn-120: Hex5HexNAc4.. Phosphorylated by Src kinases on tyrosine residues in the ITIM motif upon ligation. Interaction with TIGIT is required for Phosphorylation.

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产品实验方案

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靶点信息

Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors : CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration.. (Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.. (Microbial infection) Acts as a receptor for Pseudorabies virus.. (Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells.
See full target information PVR
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搭配使用产品

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