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AB109945

重组人PME-1蛋白

Recombinant Human PME-1 protein

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Recombinant Human PME-1 protein is a Human Full Length protein, in the 1 to 386 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

查看别名

PME1, PP2593, PRO0750, PPME1, Protein phosphatase methylesterase 1, PME-1

1 Images
SDS-PAGE - Recombinant Human PME-1 protein (AB109945)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PME-1 protein (AB109945)

15% SDS-PAGE analysis of 3μg ab109945.

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

Mass Spec, SDS-PAGE

applications

生物活性

No

访问

Q9Y570

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.058% Sodium chloride, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Previously labelled as PPME1.
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSALEKSMHLGRLPSRPPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDTFRVYKSGSEGPVLLLLHGGGHSALSWAVFTAAIISRVQCRIVALDLRSHGETKVKNPEDLSAETMAKDVGNVVEAMYGDLPPPIMLIGHSMGGAIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEEGSESISKRKKEDDMETKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGVDRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATFLIRHRFAEPIGGFQCVFPGC","proteinLength":"Full Length","predictedMolecularWeight":"44.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":386,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y570","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein phosphatase methylesterase 1 (PME-1) also known as protein phosphatase 2A methylesterase is an enzyme that regulates protein function by demethylation. PME-1 has a mass of approximately 42 kDa. It primarily resides in the cytoplasm and nucleus of cells with high expression levels in brain tissue. Its function involves the removal of methyl groups from the carboxyl-terminal leucine residue of the catalytic subunit of protein phosphatase 2A (PP2A). This action regulates the activity states of PP2A a major serine/threonine phosphatase.
Biological function summary

PME-1 influences the stability and activity of PP2A a core component of cellular signal transduction pathways. PME-1 forms part of the regulatory complexes of PP2A modulating its methylation state and associated functional properties. This regulation by PME-1 impacts cell cycle control apoptosis and other critical cellular processes. Additionally PME-1 activity affects signaling pathways by altering PP2A's interaction with various substrates and regulatory proteins.

Pathways

The role of PME-1 in cellular mechanisms links it to the Wnt signaling pathway and MAP kinase signaling cascade. PME-1 catalyzes demethylation reactions that impact PP2A's ability to associate with specific regulatory subunits. Through the Wnt pathway PME-1 affects cellular processes such as proliferation and differentiation by regulating beta-catenin activity. In relation to the MAP kinase pathway PME-1 influences cell stress response and growth factor signaling interacting with proteins like ERK and p38 MAP kinase.

PME-1 has associations with cancer and neurodegenerative conditions. In cancer altered PME-1 expression or activity can lead to uncontrolled cell growth and survival acting through PP2A-related pathways. In neurodegenerative diseases such as Alzheimer's PME-1 may affect tau protein phosphorylation by modulating PP2A activity potentially contributing to pathogenesis. As PP2A regulates tau phosphorylation disruptions in its demethylation by PME-1 has been correlated with disease progression.
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特殊说明

形式

Liquid

附加说明

ab109945 was purified using conventional chromatography.

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常规信息

功能

Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.

序列相似性

Belongs to the AB hydrolase superfamily.

翻译后修饰

Phosphorylated by SIK1 following increases in intracellular sodium, leading to dissociation from the protein phosphatase 2A (PP2A) complex and subsequent dephosphorylation of sodium/potassium-transporting ATPase ATP1A1.

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产品实验方案

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靶点信息

Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.
See full target information PPME1
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搭配使用产品

Primary Antibodies

AB205956

Anti-PME-1 antibody

Immunoprecipitation - Anti-PME-1 antibody (AB205956)

  • 0
  • 0
Primary Antibodies

AB128006

Anti-TMEPAI antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEPAI antibody (AB128006)

  • 0
  • 0
Primary Antibodies

AB183518

Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal

RabMAb|Recombinant

Western blot - Anti-DNAJC11 antibody [EPR15065(B)] - C-terminal (AB183518)

  • 0
  • 0
Primary Antibodies

AB198171

Anti-NOC2L antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOC2L antibody (AB198171)

  • 0
  • 0

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