重组人PLGF蛋白(Active) (ab174025)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人PLGF蛋白(Active)
参阅全部 PLGF 蛋白酶 -
生物活性
Measured in a cell proliferation assay using MDA - MB – 231 Human breast cancer cells.
The ED50 for this effect is typically 1 - 10 µg/ml.
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纯度
> 95 % SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
LPAVPPQQWALSAGNGSSEVEVVPFQEVWGRSYCRALERLVDVVSEYPSE VEHMFSPSCVSLLRCTGCCGDENLHCVPVETANVTMQLLKIRSGDRPSYV ELTFSQHVRCECRPLREKMKPERRRPKGRGKRRREKQRPTDCHLCGDAVP RR -
预测分子量
20 kDa including tags -
氨基酸
19 to 170 -
标签
His tag C-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab174025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
补充说明
The lyophilized product can be stored at 4°C or -80°C for 1 year. After reconstitution, store under sterile conditions for 1 month at 4°C - 8°C or 3 months at -70°C. Avoid repeated freeze-thaw cycles.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Please see notes section.
pH: 7.4
Constituents: 0.6% Acetic acid, 10% Trehalose
pH 2-3This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with 100mM acetic acid to a concentration of 200 µg/ml.
常规信息
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别名
- D12S1900
- Pgf
- PGFL
see all -
功能
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. -
组织特异性
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. -
序列相似性
Belongs to the PDGF/VEGF growth factor family. -
结构域
Isoform PlGF-2 contains a basic insert which acts as a cell retention signal. -
翻译后修饰
N-glycosylated. -
细胞定位
Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. - Information by UniProt
图片
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Functional Studies - Recombinant human PLGF protein (Active) (ab174025)
Immobilized ab174025 at 1 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1, His,Avitag (ab246160) with a linear range of 2-20 ng/mL
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Functional Studies - Recombinant human PLGF protein (Active) (ab174025)Immobilized Rhesus macaque VEGF R1, Mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind ab174025 with a linear range of 5-40 ng/mL
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SDS-PAGE - Recombinant human PLGF protein (Active) (ab174025)SDS-PAGE analysis of reduced ab174025 stained overnight with Coomassie Blue.
DTT-reduced Protein migrates as 28-33 kDa due to glycosylation.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab174025 被引用在 1 文献中.
- Xiang L et al. Placenta growth factor and vascular endothelial growth factor a have differential, cell-type specific patterns of expression in vascular cells. Microcirculation 21:368-79 (2014). PubMed: 24410720