重组人PLCZ1蛋白(ab127842)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His-DHFR tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人PLCZ1蛋白 -
纯度
> 95 % SDS-PAGE.
Purified via His tag -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
29 kDa -
氨基酸
322 to 572 -
标签
His-DHFR tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab127842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20ºC.
Constituents: 0.32% Tris HCl, 0.58% Sodium chloride
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复溶Reconstitute with water to desired concentration.
常规信息
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别名
- 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase zeta 1
- 1-phosphatidylinositol-4
- 5-bisphosphate phosphodiesterase zeta-1
see all -
功能
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca(2+)-dependent manner. Triggers intracellular Ca(2+) oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. -
组织特异性
Expressed specifically in testis. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis. -
序列相似性
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain. -
结构域
The EF-hand and C2 domains are essential for triggering Ca(2+) oscillating activity and the regulation of PLCZ1 enzyme activity.
The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization. -
细胞定位
Nucleus. Cytoplasm > perinuclear region. Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab127842 尚未被引用在任何文献中。