重组人PKC theta/PRKCQ蛋白(ab56641)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人PKC theta/PRKCQ蛋白 -
生物活性
Specific activity: 792 nmol/min/mg
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纯度
> 75 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
110 kDa -
标签
GST tag N-Terminus
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相关产品
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Related Products
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab56641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab204875 (PKC epsilon peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as PKC theta
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- KPCT_HUMAN
- MGC126514
- MGC141919
see all -
功能
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
组织特异性
Skeletal muscle, megakaryoblastic cells and platelets. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
结构域
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
翻译后修饰
Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. - Information by UniProt
图片
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Functional Studies - Recombinant human PKC theta/PRKCQ protein (ab56641)The specific activity of PKC theta/PRKCQ (ab56641) was determined to be 800 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human PKC theta/PRKCQ protein (ab56641)SDS PAGE analysis of ab56641 -
SDS-PAGE - Recombinant human PKC theta/PRKCQ protein (ab56641)SDS PAGE analysis of ab56641 -
SDS-PAGE - Recombinant human PKC theta/PRKCQ protein (ab56641)SDS Page analysis of ab56641 -
Functional Studies - Recombinant human PKC theta/PRKCQ protein (ab56641)Specific kinase activity of ab56641
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab56641 尚未被引用在任何文献中。