重组人PKC iota蛋白(ab60850)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
描述
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产品名称
重组人PKC iota蛋白 -
纯度
> 90 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
Western blot
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形式
Liquid -
补充说明
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- aPKC lambda/iota
- aPKC-lambda/iota
- Atypical protein kinase C lambda/iota
see all -
功能
Calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. May play a role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. Functions in both pro- and anti-apoptotic pathways. Functions in the RAC1/ERK signaling required for transformed growth. Plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). -
组织特异性
Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
结构域
The OPR domain mediates interaction with SQSTM1.
The C1 domain does not bind diacylglycerol (DAG). -
翻译后修饰
On neuronal growth factor (NGF) stimulation, phosphorylated by Src on Tyr-265, Tyr-280 and Tyr-334. Phosphorylation on Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation. -
细胞定位
Cytoplasm. Membrane. Endosome. Nucleus. Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by cSrc, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Vesicular tubular clusters. Transported to VTCs through interaction with RAB2A. - Information by UniProt
图片
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Functional Studies - Recombinant human PKC iota protein (ab60850)The specific activity of PKC iota (ab60850) was determined to be 572 nmol/min/mg as per activity assay protocol and was equivalent to 564 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human PKC iota protein (ab60850)SDS PAGE analysis of ab60850 -
SDS-PAGE - Recombinant human PKC iota protein (ab60850)SDS PAGE analysis of ab60850 -
Functional Studies - Recombinant human PKC iota protein (ab60850)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PKC iota protein (ab60850)ab60850 on SDS-PAGE, MW ~98 kDa. -
Western blot - Recombinant human PKC iota protein (ab60850)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab60850 尚未被引用在任何文献中。