重组人PKC delta蛋白(ab60844)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
描述
-
产品名称
重组人PKC delta蛋白 -
生物活性
Specific Activity: 302 nmol/min/mg.
-
纯度
> 90 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
标签
GST tag N-Terminus
-
相关产品
-
Related Products
-
Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Functional Studies
SDS-PAGE
Western blot
-
形式
Liquid -
补充说明
Phosphatidylinositol is the most potent activator of PKC delta.
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- CVID9
- D14Ertd420e
- Kinase PKC delta
see all -
功能
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
结构域
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner. -
翻译后修饰
Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity. -
细胞定位
Cytoplasm. Membrane. - Information by UniProt
图片
-
Functional Studies - Recombinant human PKC delta protein (ab60844)The specific activity of PKC delta (ab60844) was determined to be 250 nmol/min/mg as per activity assay protocol
-
SDS-PAGE - Recombinant human PKC delta protein (ab60844)SDS PAGE analysis of ab60844 -
SDS-PAGE - Recombinant human PKC delta protein (ab60844)SDS PAGE analysis of ab60844 -
Functional Studies - Recombinant human PKC delta protein (ab60844)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PKC delta protein (ab60844)ab60844 on SDS-PAGE, MW ~104 kDa. -
Western blot - Recombinant human PKC delta protein (ab60844)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab60844 被引用在 1 文献中.
- Lonic A et al. Phosphorylation of PKCd by FER tips the balance from EGFR degradation to recycling. J Cell Biol 220:N/A (2021). PubMed: 33411917