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重组人PKC alpha蛋白(Active) (ab55672)

Submit a review Submit a question References (5)
Functional Studies - Recombinant human PKC alpha protein (ab55672)
  • SDS-PAGE - Recombinant human PKC alpha protein (ab55672)
  • Western blot - Recombinant human PKC alpha protein (ab55672)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 95% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, WB, SDS-PAGE

描述

  • 产品名称

    重组人PKC alpha蛋白(Active)
    参阅全部 PKC alpha 蛋白酶

  • 生物活性

    Specific Activity: 3,200 nmol/min/mg.

  • 纯度

    > 95 % Densitometry.
    Affinity purified.

  • 表达系统

    Baculovirus infected Sf9 cells

  • Accession

    P17252

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Human

    • 序列

      MADVFPGNDS TASQDVANRF ARKGALRQKN VHEVKDHKFI ARFFKQPTFC SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPDTDDPRS KHKFKIHTYG SPTFCDHCGS LLYGLIHQGM KCDTCDMNVH KQCVINVPSL CGMDHTEKRG RIYLKAEVAD EKLHVTVRDA KNLIPMDPNG LSDPYVKLKL IPDPKNESKQ KTKTIRSTLN PQWNESFTFK LKPSDKDRRL SVEIWDWDRT TRNDFMGSLS FGVSELMKMP ASGWYKLLNQ EEGEYYNVPI PEGDEEGNME LRQKFEKAKL GPAGNKVISP SEDRKQPSNN LDRVKLTDFN FLMVLGKGSF GKVMLADRKG TEELYAIKIL KKDVVIQDDD VECTMVEKRV LALLDKPPFL TQLHSCFQTV DRLYFVMEYV NGGDLMYHIQ QVGKFKEPQA VFYAAEISIG LFFLHKRGII YRDLKLDNVM LDSEGHIKIA DFGMCKEHMM DGVTTRTFCG TPDYIAPEII AYQPYGKSVD WWAYGVLLYE MLAGQPPFDG EDEDELFQSI MEHNVSYPKS LSKEAVSVCK GLMTKHPAKR LGCGPEGERD VREHAFFRRI DWEKLENREI QPPFKPKVCG KGAENFDKFF TRGQPVLTPP DQLVIANIDQ SDFEGFSYVN PQFVHPILQS AV

    • 预测分子量

      103 kDa

    • 氨基酸

      1 to 672

    • 标签

      GST tag N-Terminus

    • 额外的序列信息

      (NM_002737)

相关产品

技术指标

Our Abpromise guarantee covers the use of ab55672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    Western blot

    SDS-PAGE

  • 形式

    Liquid

  • 补充说明

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity.

    For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.

    Avoid repeated handling.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride, 0.31% Glutathione

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名

    • AAG6
    • Aging associated gene 6
    • aPKC
    • KPCA_HUMAN
    • PKC alpha
    • PKC-A
    • PKC-alpha
    • PKCA
    • PRKACA
    • PRKCA
    • Protein Kinase C alpha
    • Protein kinase C alpha type
    see all

  • 功能

    This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

  • 序列相似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.

  • 细胞定位

    Cytoplasm. Cell membrane. Nucleus.
  • Information by UniProt

图片

  • Functional Studies - Recombinant human PKC alpha protein (ab55672)
    Functional Studies - Recombinant human PKC alpha protein (ab55672)

    Specific activity: 3,200 nmol/min/mg

  • SDS-PAGE - Recombinant human PKC alpha protein (ab55672)
    SDS-PAGE - Recombinant human PKC alpha protein (ab55672)

    Purity >95% by densiometry.

  • Western blot - Recombinant human PKC alpha protein (ab55672)
    Western blot - Recombinant human PKC alpha protein (ab55672)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (5)

发表研究结果有使用 ab55672？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab55672 被引用在 5 文献中.

  • Case KC  et al. Valproate regulates inositol synthesis by reducing expression of myo-inositol-3-phosphate synthase. Sci Rep 13:14844 (2023). PubMed: 37684289
  • Huang F  et al. Reversible phosphorylation of cyclin T1 promotes assembly and stability of P-TEFb. Elife 10:N/A (2021). PubMed: 34821217
  • Ahmad S  et al. Electrochemical detection of neuronal extracellular phosphorylation by PKA, PKC and Src. Anal Biochem 608:113892 (2020). PubMed: 32810472
  • Ahmad S  et al. Electrochemical studies of human nAChR a7 subunit phosphorylation by kinases PKA, PKC and Src. Anal Biochem 574:46-56 (2019). PubMed: 30914244
  • Lear TB  et al. KIAA0317 regulates pulmonary inflammation through SOCS2 degradation. JCI Insight 4:N/A (2019). PubMed: 31578312

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