重组人PKA beta (catalytic subunit)蛋白(denatured) (ab180275)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人PKA beta (catalytic subunit)蛋白(denatured)
参阅全部 PKA beta (catalytic subunit) 蛋白酶 -
纯度
> 80 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MGSSHHHHHHSSGLVPRGSHMGSMAAYREPPCNQYTGTTTALQKLEGFAS RLFHRHSKGTAHDQKTALENDSLHFSEHTALWDRSMKEFLAKAKEDFLKK WENPTQNNAGLEDFERKKTLGTGSFGRVMLVKHKATEQYYAMKILDKQKV VKLKQIEHTLNEKRILQAVNFPFLVRLEYAFKDNSNLYMVMEYVPGGEMF SHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDHQGY IQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIY EMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTK RFGNLKNGVSDIKTHKWFATTDWIAIYQRKVEAPFIPKFRGSGDTSNFDD YEEEDIRVSITEKCAKEFGEF -
预测分子量
49 kDa including tags -
氨基酸
1 to 398 -
标签
His tag N-Terminus -
额外的序列信息
NP_891993.
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描述
重组人PKA beta (catalytic subunit)蛋白
相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab180275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 2.4% Urea, 10% Glycerol (glycerin, glycerine)
常规信息
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别名
- cAMP-dependent protein kinase catalytic beta subunit isoform 4ab
- cAMP-dependent protein kinase catalytic subunit beta
- KAPCB_HUMAN
see all -
功能
Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. -
组织特异性
Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
翻译后修饰
Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively. -
细胞定位
Cytoplasm. Nucleus. Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human PKA beta (catalytic subunit) protein (denatured) (ab180275)
15% SDS-PAGE analysis of ab180275 (3μg).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab180275 尚未被引用在任何文献中。