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重组人PINK1蛋白(denatured) (ab116177)

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SDS-PAGE - Recombinant Human PINK1 protein (denatured) (ab116177)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Suitable for: SDS-PAGE

    描述

    • 产品名称

      重组人PINK1蛋白(denatured)

    • 纯度

      > 90 % SDS-PAGE.
      ab116177 was purified using conventional chromatography.

    • 表达系统

      Escherichia coli

    • Accession

      Q9BXM7

    • 蛋白长度

      Protein fragment

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 序列

        MYLIGQSIGK GCSAAVYEAT MPTLPQNLEV TKSTGLLPGR GPGTSAPGEG QERAPGAPAF PLAIKMMWNI SAGSSSEAIL NTMSQELVPA SRVALAGEYG AVTYRKSKRG PKQLAPHPNI IRVLRAFTSS VPLLPGALVD YPDVLPSRLH PEGLGHGRTL FLVMKNYPCT LRQYLCVNTP SPRLAAMMLL QLLEGVDHLV QQGIAHRDLK SDNILVELDP DGCPWLVIAD FGCCLADESI GLQLPFSSWY VDRGGNGCLM APEVSTARPG PRAVIDYSKA DAWAVGAIAY EIFGLVNPFY GQGKAHLESR SYQEAQLPAL PESVPPDVRQ LVRALLQREA SKRPSARVAA NVL

      • 预测分子量

        38 kDa

      • 氨基酸

        156 to 507

    技术指标

    Our Abpromise guarantee covers the use of ab116177 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      SDS-PAGE

    • 形式

      Liquid
    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 6.01% Urea, 0.32% Tris HCl, 5% Glycerol (glycerin, glycerine)

    常规信息

    • 别名

      • BRPK
      • FLJ27236
      • mitochondrial
      • PARK 6
      • PARK6
      • Phosphatase and Tensin Homolog
      • PINK 1
      • PINK1
      • PINK1_HUMAN
      • Protein kinase BRPK
      • PTEN induced putative kinase 1
      • PTEN induced putative kinase protein 1
      • PTEN-induced putative kinase protein 1
      • Serine/threonine kinase PINK1 mitochondrial
      • Serine/threonine protein kinase PINK1 mitochondrial
      • Serine/threonine-protein kinase PINK1
      see all

    • 功能

      Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.

    • 组织特异性

      Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.

    • 疾病相关

      Defects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.

    • 序列相似性

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.

    • 翻译后修饰

      Autophosphorylated.

    • 细胞定位

      Mitochondrion outer membrane. Cytoplasm > cytosol.
    • Information by UniProt

    图片

    • SDS-PAGE - Recombinant Human PINK1 protein (denatured) (ab116177)
      SDS-PAGE - Recombinant Human PINK1 protein (denatured) (ab116177)
      ab116177 on a 15% SDS-PAGE (3ug)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab116177？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab116177 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    Hi,
    I have a few questions about your product ab116177 which is the recombinant protein PINK1.

    How was the protein purified?

    Does it have a tag?

    Why is 1M urea in the final buffer?

    How do you know it is autophosphoryated?

    Is it active?



    Many thanks,
    Ed



    E. Wheatley PhD

    Cancer Research Technology Ltd

    Wolfson Institute for Biomedical Research

    Gower Street

    London WC1E 6BT

    Tel: (+44) 020 76790927

    Fax:(+44) 020 7796 3907

    E-mail: ewheatley@cancertechnology.com




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    Answer

    Thank you for your enquiry.

    I am pleased to help provide some answers:

    Q1. How was the protein purified? I am sorry to confirm that this particular product is sourced externally and we regrettably are not able to share manufacturing process information on this occasion.

    Q2. Does it have a tag? No, it does not have a tag.

    Q3. Why is 1 M urea in the final buffer? The urea helps prevent aggregation.

    Q4. Do you know if the protein is phosphorylated? If so, how has this been assessed? We have not confirmed if this protein is phosphorylated, but we suspect it isn’t. It’s most likely denatured.

    Q5. Is the protein active? I am sorry this has not been tested. It is unlikely to be active if it is denatured.

    I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.

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