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生物活性等级

重组人Pin1蛋白(ab51230)

Submit a review Q&A (2)References (2)
SDS-PAGE - Recombinant human Pin1 protein (ab51230)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Suitable for: Functional Studies, SDS-PAGE

    描述

    • 产品名称

      重组人Pin1蛋白
      参阅全部 Pin1 蛋白酶

    • 生物活性

      Specific activity is > 1,200nmol/min/mg, and is defined as the amount of enzyme that cleaves of suc-AAPF-pNA per minute at 37C.

    • 纯度

      > 95 % SDS-PAGE.

    • 内毒素水平

      < 1.000 Eu/µg

    • 表达系统

      Escherichia coli

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Human

      • 序列

        MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQG EPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGE EDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPV FTDSGIHIILRTE

    技术指标

    Our Abpromise guarantee covers the use of ab51230 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      Functional Studies

      SDS-PAGE

    • 质谱法

      MALDI-TOF

    • 形式

      Liquid
    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.50
      Constituents: 0.077% DTT, 0.316% Tris HCl, 20% Glycerol, 0.58% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    常规信息

    • 别名

      • DOD
      • DODO, Drosophila, homolog of
      • FLJ40239
      • FLJ77628
      • MGC10717
      • NIMA interacting 1
      • Peptidyl prolyl cis trans isomerase NIMA interacting 1
      • Peptidyl prolyl cis/trans isomerase NIMA interacting
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
      • Peptidyl-prolyl cis-trans isomerase pin1
      • Peptidylprolyl cis/trans isomerase NIMA interacting 1
      • Pin 1
      • Pin1
      • PIN1_HUMAN
      • PPIase Pin1
      • Prolyl isomerase
      • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1
      • Protein NIMA interacting 1
      • Rotamase Pin1
      • UBL 5
      • UBL5
      see all

    • 功能

      Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.

    • 序列相似性

      Contains 1 PpiC domain.
      Contains 1 WW domain.

    • 结构域

      The WW domain is required for the interaction with STIL and KIF20B.

    • 翻译后修饰

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    图片

    • SDS-PAGE - Recombinant human Pin1 protein (ab51230)
      SDS-PAGE - Recombinant human Pin1 protein (ab51230)
      ab51230 run on a 14% SDS-PAGE gel with molecular weight markers.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (2)

    发表研究结果有使用 ab51230？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab51230 被引用在 2 文献中.

    • Leung JK  et al. Pin1 inhibition improves the efficacy of ralaniten compounds that bind to the N-terminal domain of androgen receptor. Commun Biol 4:381 (2021). PubMed: 33753863
    • Labrador-Garrido A  et al. Chaperome screening leads to identification of Grp94/Gp96 and FKBP4/52 as modulators of the a-synuclein-elicited immune response. FASEB J 30:564-77 (2016). PubMed: 26443817

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    I have recently ordered Rec. Pin1 (Active) (Ab51230) and would like to dilute the protein 1:10 and aliquot and freezing at -80. What would you recommend that I reconstitute the protein in for freezing and for retaining the activity of Pin1?

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    Answer

    You can dilute the protein in the same storage buffer in which the product is received. This buffer contains:
    Preservative: None
    Constituents: 20% Glycerol, 0.1M Sodium chloride, 5mM DTT, 20mM Tris HCl, pH 7.5
    Please remember to keep freeze/thaws to a minimum.

    Read More

    Question

    In the datasheet for product ab51230 (Pin1 protein) there is a section on biological activity. Does the product come with an activity assay? If not, do you sell the buffer and substrate for the activity assay?

    Read More
    Answer

    The product ab51230 does not come with an assay kit and we unfortunately do not have one in our catalogue. If you follow the instructions on the website though, you should be able to perform the assay.

    Read More

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