JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB204146

重组人PIM3蛋白

Recombinant human PIM3 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human PIM3 protein is a Human Full Length protein, in the 1 to 326 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

查看别名

Serine/threonine-protein kinase pim-3, PIM3

2 Images
Functional Studies - Recombinant human PIM3 protein (AB204146)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PIM3 protein (AB204146)

Sample Kinase Activity Plot. ab204146 specific activity was determined to be 900 nmol/min/mg.

SDS-PAGE - Recombinant human PIM3 protein (AB204146)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PIM3 protein (AB204146)

SDS-PAGE analysis of ab204146. MW 65 KDa.

关键信息

纯度

>90% Densitometry

表达系统

Baculovirus infected Sf9 cells

标签

GST tag N-Terminus

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

The specific activity is 900 mol/min/mg.

访问

Q86V86

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

ab204879 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
style
left-column

序列信息

[{"sequence":"MLLSKFGSLAHLCGPGGVDHLPVKILQPAKADKESFEKAYQVGAVLGSGGFGTVYAGSRIADGLPVAVKHVVKERVTEWGSLGGATVPLEVVLLRKVGAAGGARGVIRLLDWFERPDGFLLVLERPEPAQDLFDFITERGALDEPLARRFFAQVLAAVRHCHSCGVVHRDIKDENLLVDLRSGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLLFRRRVSPECQQLIRWCLSLRPSERPSLDQIAAHPWMLGADGGVPESCDLRLCTLDPDDVASTTSSSESL","proteinLength":"Full Length","predictedMolecularWeight":"65 kDa","actualMolecularWeight":null,"aminoAcidEnd":326,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q86V86","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The PIM3 protein is a member of the proviral integration site for moloney murine leukemia virus (PIM) serine/threonine kinase family. It is also known as PIM-3 kinase. This protein has a mass of approximately 37 kDa. Expression of PIM3 is found in various tissues including liver pancreas kidney and some cancer cell lines. Scientists recognize its role in phosphorylating target proteins which affects various cellular processes like cell cycle and apoptosis.
Biological function summary

PIM3 functions by regulating cell survival and proliferation. It does not form part of any large protein complex but operates through phosphorylation of substrates. This kinase modulates signaling pathways important for mitogenic stimuli and cellular responses to various stress factors. Tissues with abnormal PIM3 levels may experience altered cell growth and survival which can affect normal physiological processes.

Pathways

PIM3 interacts with cellular signaling networks. It is notably involved in the PI3K/AKT signaling pathway affecting cell growth and metabolism. Additionally PIM3 participates in mTOR signaling linking it to protein synthesis and cellular energy balance. It shows functional relation with proteins such as AKT and mTOR suggesting its integration in these pivotal regulatory circuits.

Excessive PIM3 expression associates with certain types of cancer such as pancreatic cancer and hepatocellular carcinoma. It contributes to oncogenic processes by promoting uncontrolled cell growth and survival. In cancer contexts PIM3 interacts with oncogenes like c-MYC which may exacerbate tumorous characteristics. Targeting PIM3 pathways offers potential therapeutic strategies for related malignancies.
style
left-column

特殊说明

形式

Liquid

附加说明

Affinity purified.

style
left-column

常规信息

功能

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through : the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.

序列相似性

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.

翻译后修饰

Ubiquitinated, leading to proteasomal degradation.. Phosphorylated. Interaction with PPP2CA promotes dephosphorylation.

style
left-column

产品实验方案

style
left-column

靶点信息

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through : the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.
See full target information PIM3
style
left-column
style
left-column
style
right-column

搭配使用产品

Primary Antibodies

AB198842

Anti-PIM3 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIM3 antibody - C-terminal (AB198842)

  • 0
  • 0
Biochemicals

AB141591

STO-609 acetate, CaM-KK inhibitor

Chemical Structure - STO-609 acetate, CaM-KK inhibitor (AB141591)

  • 0
  • 0
Proteins & Peptides

AB235719

Recombinant Human GLP-1 protein (Tagged)

SDS-PAGE - Recombinant Human GLP-1 protein (Tagged) (AB235719)

  • 0
  • 0
Proteins & Peptides

AB105593

Recombinant Human Iba1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Iba1 protein (His tag N-Terminus) (AB105593)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com