重组人PIM2蛋白(ab60836)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人PIM2蛋白
参阅全部 PIM2 蛋白酶 -
纯度
> 95 % SDS-PAGE.
Purity: >95% as determined by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
标签
GST tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab60836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab204880 (RPS6 peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- OTTHUMP00000025825
- Pim 2 oncogene
- Pim 2h
see all -
功能
Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate. -
组织特异性
Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. - Information by UniProt
图片
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Functional Studies - Recombinant human PIM2 protein (ab60836)The specific activity of PIM2 (ab60836) was determined to be 270 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human PIM2 protein (ab60836)SDS PAGE analysis of ab60836 -
Functional Studies - Recombinant human PIM2 protein (ab60836)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PIM2 protein (ab60836)ab60836 on SDS-PAGE, MW ~61kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab60836 尚未被引用在任何文献中。