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AB95857

重组人 PIG3 蛋白 (His tag N-Terminus)

Recombinant Human PIG3 protein (His tag N-Terminus)

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Recombinant Human PIG3 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 332 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

查看别名

PIG3, TP53I3, Quinone oxidoreductase PIG3, NADPH:quinone reductase PIG3, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein, Protein PIG3

1 Images
SDS-PAGE - Recombinant Human PIG3 protein (His tag N-Terminus) (AB95857)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PIG3 protein (His tag N-Terminus) (AB95857)

15% SDS-PAGE analysis of 3μg ab95857.

关键信息

纯度

>90% SDS-PAGE

ab95857 is purified using conventional chromatography techniques.

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

Mass Spec, SDS-PAGE

applications

生物活性

No

访问

Q53FA7

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl

storage-buffer

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搭配使用产品

Primary Antibodies

AB96819

Anti-PIG3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PIG3 antibody (AB96819)

  • 0
  • 0
Primary Antibodies

AB121821

Anti-MRPL41 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRPL41 antibody (AB121821)

  • 0
  • 0
Primary Antibodies

AB70335

Anti-nmt55 / p54nrb antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-nmt55 / p54nrb antibody (AB70335)

  • 4
  • 5
Primary Antibodies

AB177149

Anti-SFPQ antibody [EPR11847]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (AB177149)

  • 5
  • 3
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMLAVHFDKPGGPENLYVKEVAKPSPGEGEVLLKVAASALNRADLMQRQGQYDPPPGASNILGLEASGHVAELGPGCQGHWKIGDTAMALLPGGGQAQYVTVPEGLLMPIPEGLTLTQAAAIPEAWLTAFQLLHLVGNVQAGDYVLIHAGLSGVGTAAIQLTRMAGAIPLVTAGSQKKLQMAEKLGAAAGFNYKKEDFSEATLKFTKGAGVNLILDCIGGSYWEKNVNCLALDGRWVLYGLMGGGDINGPLFSKLLFKRGSLITSLLRSRDNKYKQMLVNAFTEQILPHFSTEGPQRLLPVLDRIYPVTEIQEAHKYMEANKNIGKIVLELPQ","proteinLength":"Full Length","predictedMolecularWeight":"37.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":332,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q53FA7","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PIG3 also known as PIG00 or P@IG3 str0h is a protein linked to cellular stress response with a molecular mass of approximately 55 kDa. It shows expression mainly in tissues that undergo oxidative stress or DNA damage acting as an important player in apoptosis. PIG3 is mainly known as p53-induced gene 3 aligning with its dependency on p53 for activation. High expression levels become evident in tissues encountering high metabolic or environmental stress.
Biological function summary

PIG3 mediates cell apoptosis in response to DNA damage and oxidative stress. It forms part of a complex with p53 contributing to the regulation of apoptotic pathways. As an oxidoreductase PIG3 initiates pro-apoptotic changes within the cell. Its enzymatic activity involves producing reactive oxygen species (ROS) further amplifying the cell’s apoptotic initiations. Its role connects to cellular response mechanisms in stress conditions dealing with damaged or unhealthy cells.

Pathways

PIG3 operates within the important p53 apoptotic pathway and the DNA damage signaling pathway. It significantly interacts with p53 as PIG3 expression depends on p53 transcription factor activation. Also PIG3 connects with caspase pathways by influencing the downstream apoptosis cascade required for cell death. It acts as a link bridging initial DNA damage response and the execution of apoptosis providing a control node within these pathways.

Research links PIG3 to cancer and neurodegenerative diseases. In cancer its expression may alter affecting cell apoptosis and proliferation balance and interacting closely with proteins like p53 and BAX. In neurodegenerative disorders abnormal PIG3 activity couples with oxidative stress that leads to neuronal degeneration. Its interplay with p53 remains significant as p53's regulation of apoptotic pathways impacts numerous conditions where PIG3's role becomes equally affected.
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常规信息

功能

NADPH : quinone reductase which expression is induced by p53/TP53 and is involved in both the DNA damage response through homologous recombination repair, and p53-mediated apoptosis (PubMed : 19349281, PubMed : 33782397). Catalyzes the NADPH-dependent reduction of quinones, exhibiting a low enzymatic activity with beta-naphthoquinones and a strong preference for the ortho-quinone isomer (1,2-beta-naphthoquinone) over the para isomer (1,4-beta-naphthoquinone). Also displays a low reductase activity for non-quinone compounds such as diamine and 2,6-dichloroindophenol (in vitro) (PubMed : 19349281). The reduction of 1,2-naphthoquinone concurrently generates reactive oxygen species (ROS), which may trigger apoptosis downstream of p53/TP53 (PubMed : 19349281).

序列相似性

Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.

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产品实验方案

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靶点信息

NADPH : quinone reductase which expression is induced by p53/TP53 and is involved in both the DNA damage response through homologous recombination repair, and p53-mediated apoptosis (PubMed : 19349281, PubMed : 33782397). Catalyzes the NADPH-dependent reduction of quinones, exhibiting a low enzymatic activity with beta-naphthoquinones and a strong preference for the ortho-quinone isomer (1,2-beta-naphthoquinone) over the para isomer (1,4-beta-naphthoquinone). Also displays a low reductase activity for non-quinone compounds such as diamine and 2,6-dichloroindophenol (in vitro) (PubMed : 19349281). The reduction of 1,2-naphthoquinone concurrently generates reactive oxygen species (ROS), which may trigger apoptosis downstream of p53/TP53 (PubMed : 19349281).
See full target information TP53I3
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