重组人PI3K (p110 delta/p85 alpha)蛋白(Active) (ab125633)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: His tag N-Terminus, His tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
重组人PI3K (p110 delta/p85 alpha)蛋白(Active) -
生物活性
The specific activity of ab125633 was equivalent to 2,514 nmol/min/mg as per activity assay protocol.
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纯度
> 75 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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氨基酸序列 1
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种属
Human -
预测分子量
111 kDa -
氨基酸
1 to 1044 -
标签
His tag N-Terminus -
额外的序列信息
NM_005026.
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氨基酸序列 2
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种属
Human -
预测分子量
86 kDa -
氨基酸
2 to 724 -
标签
His tag N-Terminus -
额外的序列信息
NM_181253.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab125633 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
Kinase assay.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.002% PMSF, 0.82% Sodium phosphate, 0.004% DTT, 25% Glycerol, 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
图片
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Functional Studies - Recombinant human PI3K (p110 delta/p85 alpha) protein (Active) (ab125633)
The specific activity of ab125633 was equivalent to 2,514 nmol/min/mg as per activity assay protocol.
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SDS-PAGE - Recombinant human PI3K (p110 delta/p85 alpha) protein (Active) (ab125633)Purity of ab125633 was determined to be >75% by densiometry.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab125633 被引用在 8 文献中.
- Cai M & Sheng L MFG-E8 Exerts Neuroprotection in Neural Stem Cells Induced by Anesthetic Sevoflurane via Regulating the PI3K/AKT Pathways. Stem Cells Int 2022:5609501 (2022). PubMed: 36277041
- Tan L et al. LncRNA GAS5 as miR-26a-5p Sponge Regulates the PTEN/PI3K/Akt Axis and Affects Extracellular Matrix Synthesis in Degenerative Nucleus Pulposus Cells in vitro. Front Neurol 12:653341 (2021). PubMed: 34413821
- Chen Q et al. LncRNA LINC00641 Sponges miR-497-5p to Ameliorate Neural Injury Induced by Anesthesia via Up-Regulating BDNF. Front Mol Neurosci 13:95 (2020). PubMed: 32714145
- Cattley RT et al. Transforming growth factor ß (TGF-ß) receptor signaling regulates kinase networks and phosphatidylinositol metabolism during T-cell activation. J Biol Chem 295:8236-8251 (2020). PubMed: 32358062
- Xu J et al. Knockdown of serpin peptidase inhibitor clade C member 1 inhibits the growth of nasopharyngeal carcinoma cells. Mol Med Rep 19:3658-3666 (2019). PubMed: 30896875
- Liu Y et al. Alisol B 23-acetate inhibits the viability and induces apoptosis of non-small cell lung cancer cells via PI3K/AKT/mTOR signal pathway. Mol Med Rep 20:1187-1195 (2019). PubMed: 31173235
- Shi X et al. Overexpression of Long Noncoding RNA PTENP1 Inhibits Cell Proliferation and Migration via Suppression of miR-19b in Breast Cancer Cells. Oncol Res 26:869-878 (2018). PubMed: 29212574
- Zhang H et al. Inhibition of the PI3K/Akt signaling pathway reverses sorafenib-derived chemo-resistance in hepatocellular carcinoma. Oncol Lett 15:9377-9384 (2018). PubMed: 29928334