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AB211320

重组人 PGK1 蛋白 (His tag N-Terminus)

Recombinant human PGK1 protein (His tag N-Terminus)

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(1 Publication)

Recombinant human PGK1 protein is a Human Full Length protein, in the 1 to 417 aa range with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies and mass spectrometry.The predicted molecular weight of ab211320 protein is 46.8 kDa.

- Save time and ensure accurate results - use our recombinant Human Phosphoglycerate kinase-1 (PGK-1/ PGKA) protein as a control
- Optimal protein bioactivity and stability

查看别名

PGKA, MIG10, OK/SW-cl.110, PGK1, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2

1 Images
SDS-PAGE - Recombinant human PGK1 protein (His tag N-Terminus) (AB211320)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PGK1 protein (His tag N-Terminus) (AB211320)

15% SDS-PAGE analysis of ab211320 (3μg).

关键信息

纯度

>95% SDS-PAGE

ab211320 was purified using conventional chromatography techniques.

内毒素水平

< 1 EU/µg

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

FuncS, Mass Spec, SDS-PAGE

applications

生物活性

Yes

生物学活性

Specific activity: > 600 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37°C.

访问

P00558

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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Sample Prep & Detection Kits

AB236553

Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link®

Immunocytochemistry - Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (AB236553)

  • 5
  • 5
Proteins & Peptides

AB224782

Recombinant human Triosephosphate isomerase protein (Active) (His tag N-Terminus)

SDS-PAGE - Recombinant human Triosephosphate isomerase protein (Active) (His tag N-Terminus) (AB224782)

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Primary Antibodies

AB199438

Anti-PGK1 antibody [EPR19057]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PGK1 antibody [EPR19057] (AB199438)

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  • 2
Proteins & Peptides

AB167888

Recombinant E. coli Maltose Binding Protein (His tag N-Terminus)

SDS-PAGE - Recombinant E. coli Maltose Binding Protein (His tag N-Terminus) (AB167888)

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  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Ensure the validity of your result using our bioactive recombinant human PGK-1/ PGKA as a control in SDS-PAGE and mass spectrometry.


Check out our protein gel staining guide for SDS-PAGE here
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序列信息

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI","proteinLength":"Full Length","predictedMolecularWeight":"46.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":417,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00558","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PGK1 also known as phosphoglycerate kinase-1 is an enzyme involved in glycolysis with a mass of approximately 45 kDa. PGK1 catalyzes the reversible transfer of a phosphate group from 13-bisphosphoglycerate to ADP forming 3-phosphoglycerate and ATP. This enzyme is expressed ubiquitously across various tissues with significant roles in energy metabolism. In yeast it is referred to simply as phosphoglycerate kinase where its function in cellular respiration is widely studied.
Biological function summary

The PGK1 protein plays an essential role in energy production by participating in the glycolytic pathway. This enzyme functions independently and is not part of any complex. By converting 13-bisphosphoglycerate to 3-phosphoglycerate it supports cellular processes that require ATP. Such activity facilitates the high energy needs of cells ensuring proper cellular function and homeostasis.

Pathways

The enzyme PGK1 operates predominantly within the glycolytic and gluconeogenesis pathways. Throughout glycolysis it partners with enzymes like phosphofructokinase and pyruvate kinase facilitating the breakdown of glucose into pyruvate while generating ATP. In gluconeogenesis PGK1 aids in glucose formation which helps balance energy dynamics particularly in tissues like the liver and kidney.

PGK1 has associations with tumor growth and proliferation in various cancers. Its elevated expression is often found in tumors linking it to oncogenesis due to the increased demand for glycolytic activity. Additionally mutations in PGK1 can result in phosphoglycerate kinase deficiency a disorder affecting red blood cell metabolism. This links it to related proteins involved in managing metabolic pathways of energy production in affected tissues.
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常规信息

功能

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed : 30323285, PubMed : 7391028). Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines (PubMed : 18463139). In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed : 2324090). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl-coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis (PubMed : 26942675, PubMed : 36849569). May play a role in sperm motility (PubMed : 26677959).

序列相似性

Belongs to the phosphoglycerate kinase family.

翻译后修饰

Phosphorylated at Ser-203 by MAPK1/ERK2 under hypoxic conditions, which promotes its mitochondrial targeting.

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产品实验方案

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靶点信息

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed : 30323285, PubMed : 7391028). Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines (PubMed : 18463139). In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed : 2324090). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl-coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis (PubMed : 26942675, PubMed : 36849569). May play a role in sperm motility (PubMed : 26677959).
See full target information PGK1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Neoplasia (New York, N.Y.) 21:893-907 PubMed31401411

2019

A Novel Pyrazolopyrimidine Ligand of Human PGK1 and Stress Sensor DJ1 Modulates the Shelterin Complex and Telomere Length Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Alan E Bilsland,Yu Liu,Andrew Turnbull,David Sumpton,Katrina Stevenson,Claire J Cairney,Susan M Boyd,Jon Roffey,David Jenkinson,W Nicol Keith
View all publications
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