重组人Peroxiredoxin 2/PRP蛋白(ab79947)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
描述
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产品名称
重组人Peroxiredoxin 2/PRP蛋白
参阅全部 Peroxiredoxin 2/PRP 蛋白酶 -
生物活性
Specific Activity: 149 pmol/min/µg. 3.45 µg of Peroxiredoxin 2 was incubated in 50 mM Hepes (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.
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纯度
> 95 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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技术指标
Our Abpromise guarantee covers the use of ab79947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
Western blot
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Epididymis secretory sperm binding protein Li 2a
- HEL S 2a
- MGC4104
see all -
功能
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). -
序列相似性
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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Functional Studies - Recombinant human Peroxiredoxin 2/PRP protein (ab79947)
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SDS-PAGE - Recombinant human Peroxiredoxin 2/PRP protein (ab79947)Lane 1: ab79947 on 14% SDS-PAGE, Coomassie staining, 10µg.
Lane 2: Protein marker. -
Western blot - Recombinant human Peroxiredoxin 2/PRP protein (ab79947)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab79947 尚未被引用在任何文献中。