重组人PDPK1蛋白(ab60834)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
描述
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产品名称
重组人PDPK1蛋白 -
生物活性
Specific Activity: 123 nmol/min/mg.
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纯度
> 80 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab60834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
Western blot
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形式
Liquid -
补充说明
ab204873 (AKT1 + PKN2 peptide substrate) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 3 phosphoinositide dependent protein kinase 1
- 3-phosphoinositide-dependent protein kinase 1
- hPDK 1
see all -
功能
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive. -
组织特异性
Appears to be expressed ubiquitously. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. -
细胞定位
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. - Information by UniProt
图片
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SDS-PAGE - Recombinant human PDPK1 protein (ab60834)
SDS PAGE analysis of ab60834.
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Functional Studies - Recombinant human PDPK1 protein (ab60834)The specific activity of PDPK1 (ab60834) was determined to be 25 nmol /min/mg as per activity assay protocol.
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Functional Studies - Recombinant human PDPK1 protein (ab60834)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PDPK1 protein (ab60834)ab60834 on SDS-PAGE, MW ~67kDa. -
Western blot - Recombinant human PDPK1 protein (ab60834)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab60834 被引用在 3 文献中.
- Chen M et al. A p53-phosphoinositide signalosome regulates nuclear AKT activation. Nat Cell Biol 24:1099-1113 (2022). PubMed: 35798843
- Stangherlin A et al. Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology. Nat Commun 12:6035 (2021). PubMed: 34654800
- Li X et al. Cyanidin inhibits EMT induced by oxaliplatin via targeting the PDK1-PI3K/Akt signaling pathway. Food Funct 10:592-601 (2019). PubMed: 30672917