重组人PDK4蛋白(ab125560)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
重组人PDK4蛋白
参阅全部 PDK4 蛋白酶 -
生物活性
The specific activity of ab125560 is 20 nmol/min/mg.
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纯度
> 90 % Densitometry.
ab125560 is >90% pure. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
71 kDa -
氨基酸
1 to 411 -
标签
GST tag N-Terminus
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相关产品
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Related Products
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab125560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.004% DTT, 0.002% PMSF, 0.31% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4
- FLJ40832
- mitochondrial
see all -
功能
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
组织特异性
Ubiquitous; highest levels of expression in heart and skeletal muscle. -
序列相似性
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
细胞定位
Mitochondrion matrix. - Information by UniProt
图片
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Functional Studies - Recombinant human PDK4 protein (ab125560)The specific activity of ab125560 was determined to be 20 nmol/min/mg.
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SDS-PAGE - Recombinant human PDK4 protein (ab125560)SDS PAGE analysis of ab125560 (PDK4 protein).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab125560 被引用在 1 文献中.
- Ma Z et al. PDK4 facilitates fibroblast functions and diabetic wound healing through regulation of HIF-1α protein stability and gene expression. FASEB J 37:e23215 (2023). PubMed: 37737961