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AB152601

重组人 PDHB 蛋白 (GST tag N-Terminus)

Recombinant Human PDHB protein (GST tag N-Terminus)

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Recombinant Human PDHB protein (GST tag N-Terminus) is a Human Fragment protein, in the 250 to 359 aa range, expressed in Wheat germ, suitable for ELISA, WB.

查看别名

PHE1B, PDHB, PDHE1-B

1 Images
SDS-PAGE - Recombinant Human PDHB protein (GST tag N-Terminus) (AB152601)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PDHB protein (GST tag N-Terminus) (AB152601)

ab152601 on a 12.5% SDS-PAGE stained with Coomassie Blue.

关键信息

表达系统

Wheat germ

标签

GST tag N-Terminus

应用

ELISA, WB

applications

生物活性

No

访问

P11177

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

This product was previously labelled as Pyruvate Dehydrogenase E1 beta subunit.
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序列信息

[{"sequence":"LEAAAVLSKEGVECEVINMRTIRPMDMETIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSIPQVKDIIFAIKKTLNI","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":359,"aminoAcidStart":250,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P11177","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PDHB also known as pyruvate dehydrogenase beta subunit plays an important role in the conversion of pyruvate to acetyl-CoA. This protein forms a part of the pyruvate dehydrogenase complex (PDC) which occurs in mitochondria of numerous tissues. The PDHB subunit has a molecular mass of approximately 39 kDa. Expression of PDHB is not limited to one specific type of tissue it is generally found in various tissues indicating its widespread involvement in cellular metabolism.
Biological function summary

PDHB functions as an essential component of the PDC which catalyzes the decarboxylation of pyruvate linking glycolysis to the citric acid cycle. The PDC itself is a large multi-enzyme complex including other subunits such as E1 and E2 which coordinate efficiently to carry out its function. In this way PDHB contributes to energy production by enabling entry of carbon skeletons into the tricarboxylic acid cycle important for ATP generation.

Pathways

PDHB holds a significant role in central metabolic pathways namely glycolysis and the citric acid cycle. The conversion facilitated by the PDC forms a vital cornerstone for metabolic flux regulation influencing gluconeogenesis and fatty acid synthesis. PDHB also interacts with proteins such as pyruvate dehydrogenase kinase which regulates the PDC activity by phosphorylation thereby affecting energy metabolism directly.

Alterations in PDHB can lead to metabolic disorders such as pyruvate dehydrogenase deficiency potentially causing neurological dysfunction due to impaired energy metabolism. PDHB mutations also have a link to mitochondrial diseases reflecting its significant role in energy homeostasis. In these contexts proteins like the pyruvate dehydrogenase phosphatase are involved highlighting the clinical relevance of PDHB alterations in maintaining proper metabolic health.
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特殊说明

形式

Liquid

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常规信息

功能

Together with PDHA1 forms the heterotetrameric E1 subunit of the pyruvate dehydrogenase (PDH) complex (PubMed : 17474719, PubMed : 19081061). The PDH complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable). It contains multiple copies of three enzymatic components : pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3) (Probable). The E1 subunit catalyzes both the thiamine pyrophosphate (TPP)-dependent decarboxylation of pyruvate and the reductive acetylation of a lipoyl group covalently linked to the lipoyl-bearing domains of E2 (PubMed : 19081061).

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产品实验方案

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靶点信息

Together with PDHA1 forms the heterotetrameric E1 subunit of the pyruvate dehydrogenase (PDH) complex (PubMed : 17474719, PubMed : 19081061). The PDH complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable). It contains multiple copies of three enzymatic components : pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3) (Probable). The E1 subunit catalyzes both the thiamine pyrophosphate (TPP)-dependent decarboxylation of pyruvate and the reductive acetylation of a lipoyl group covalently linked to the lipoyl-bearing domains of E2 (PubMed : 19081061).
See full target information PDHB
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搭配使用产品

Primary Antibodies

AB155996

Anti-PDHB antibody [EPR11097(B)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHB antibody [EPR11097(B)] (AB155996)

  • 0
  • 0
Proteins & Peptides

AB114378

Recombinant Human Aconitase 2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Aconitase 2 protein (GST tag N-Terminus) (AB114378)

  • 0
  • 0
Primary Antibodies

AB110316

Anti-ETFA antibody [2B11AE8]

BOND RX™ Validated|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-ETFA antibody [2B11AE8] (AB110316)

  • 0
  • 0
Proteins & Peptides

AB316432

Recombinant Human MASP2 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human MASP2 Protein Standard (His tag) (AB316432)

  • 0
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