重组人PDGF AB蛋白(Animal Free) (ab179496)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
-
产品名称
重组人PDGF AB蛋白(Animal Free) -
生物活性
The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically less than 1 ng/mL.
-
纯度
> 95 % SDS-PAGE.
Purity determined by Reducing and Non-reducing SDS-PAGE. -
内毒素水平
<=1.000 Eu/µg -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
Yes -
性质
Recombinant -
-
种属
Human -
序列
Alpha chain: MSIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVK RCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLEC ACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT Beta chain: SLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPP CVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIGIVRKKPIFKKATVTLG DHLACKCETVAAARPVT -
预测分子量
26 kDa -
额外的序列信息
Non-glycosylated, disulfide-linked heterodimer, containing one 14.3 kDa alpha-chain (aa87-211; UniProt P04085) and one 12.1 kDa beta-chain (aa82-190; UniProt P01127).
-
技术指标
Our Abpromise guarantee covers the use of ab179496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
SDS-PAGE
Functional Studies
-
形式
Lyophilized -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
Constituent: 0.06% Acetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
复溶Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilised product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
常规信息
-
别名
- becaplermin
- c-sis
- GDGF
see all -
相关性
Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains joined by disulphide bonds, termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain. -
细胞定位
Secreted
图片
-
Functional Studies - Recombinant human PDGF AB protein (Animal Free) (ab179496)Functional analysis of ab179496
-
SDS-PAGE - Recombinant human PDGF AB protein (Animal Free) (ab179496)SDS PAGE analysis of ab179496 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab179496 尚未被引用在任何文献中。