重组人PDE7A/HCP1蛋白(ab125786)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人PDE7A/HCP1蛋白
参阅全部 PDE7A/HCP1 蛋白酶 -
生物活性
The specific activity of ab125786 was determined to be 166 nmol/min/mg.
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纯度
> 85 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSSNSL NILDDDYNGQ AKCMLEKVGN WNFDIFLFDR LTNGNSLVSL TFHLFSLHGL IEYFHLDMMK LRRFLVMIQE DYHSQNPYHN AVHAADVTQA MHCYLKEPKL ANSVTPWDIL LSLIAAATHD LDHPGVNQPF LIKTNHYLAT LYKNTSVLEN HHWRSAVGLL RESGLFSHLP LESRQQMETQ IGALILATDI SRQNEYLSLF RSHLDRGDLC LEDTRHRHLV LQMALKCADI CNPCRTWELS KQWSEKVTEE FFHQGDIEKK YHLGVSPLCD RHTESIANIQ IGFMTYLVEP LFTEWARFSN TRLSQTMLGH VGLNKASWKG LQREQSSSED TDAAFELNSQ LLPQENRLS -
预测分子量
64 kDa including tags -
氨基酸
104 to 456 -
标签
GST tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab125786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
This product was previously labelled as PDE7A
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.002% PMSF, 0.4% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 5''-cyclic phosphodiesterase 7A
- HCP 1
- HCP1
see all -
功能
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction. -
组织特异性
PDE7A1 is found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart. It is expressed as well in two T-cell lines. PDE7A2 is found abundantly in skeletal muscle and at low levels in heart. -
通路
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily. -
发展阶段
Developmentally regulated. PDE7A1 and PDE7A2 are found in several fetal tissues, expression is reduced throughout development. It persists strongly only in adult skeletal muscle. -
结构域
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain. -
细胞定位
Cytoplasm > cytosol. PDE7A1 (57 kDa) is located mostly to soluble cellular fractions and Cytoplasm. PDE7A2 (50 kDa) is located to particulate cellular fractions. - Information by UniProt
图片
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Functional Studies - Recombinant human PDE7A/HCP1 protein (ab125786)The specific activity of PDE7A/HCP1 (ab125786) was determined to be 160 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human PDE7A/HCP1 protein (ab125786)SDS PAGE analysis of ab125786 -
SDS-PAGE - Recombinant human PDE7A/HCP1 protein (ab125786)SDS PAGE analysis of ab125786 -
SDS-PAGE - Recombinant human PDE7A/HCP1 protein (ab125786)SDS-PAGE analysis of ab125786. -
Functional Studies - Recombinant human PDE7A/HCP1 protein (ab125786)Sample assay showing the specific activity of ab125786 to be 166 nmol/min/mg.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125786 尚未被引用在任何文献中。