重组人PDE5A/PDE5蛋白(ab56614)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 40% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies
描述
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产品名称
重组人PDE5A/PDE5蛋白
参阅全部 PDE5A/PDE5 蛋白酶 -
生物活性
Specific Activity: ≥ 3,100 pmole/min/µg
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纯度
> 40 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
标签
GST tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab56614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
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形式
Liquid -
补充说明
This product was previously labelled as PDE5A
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.1535% Glutathione, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 5''-cyclic phosphodiesterase
- CGB PDE
- CGB-PDE
see all -
功能
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. -
组织特异性
Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung. -
通路
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. -
序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
结构域
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B. -
翻译后修饰
Phosphorylation is regulated by binding of cGMP to the two allosteric sites. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab56614 尚未被引用在任何文献中。