重组人PD-L1蛋白(Active) (ab280943)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: MS, Sandwich ELISA, SDS-PAGE, HPLC, Functional Studies
描述
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产品名称
重组人PD-L1蛋白(Active)
参阅全部 PD-L1 蛋白酶 -
生物活性
Fully biologically active measured by its ability to inhibit anti-CD3 and anti-CD8 antibody induced T-cell expansion. ED50 for this effect is ≤500 ng/mL with a specific activity of 0.2 x 104 units/mg.
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纯度
>= 95 % SDS-PAGE.
=95% by HPLC -
内毒素水平
<=0.005 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
Yes -
无载体
是 -
性质
Recombinant -
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种属
Human -
序列
FTVTVPKDLY VVEYGSNMTI ECKFPVEKQL DLAALIVYWE MEDKNIIQFV HGEEDLKVQH SSYRQRARLL KDQLSLGNAA LQITDVKLQD AGVYRCMISY GGADYKRITV KVNAPYNKIN QRILVVDPVT SEHELTCQAE GYPKAEVIWT SSDHQVLSGK TTTTNSKREE KLFNVTSTLR INTTTNEIFY CTFRRLDPEE NHTAELVIPE LPLAHPPNER -
预测分子量
25 kDa -
实际分子量
25 kDa -
分子量信息
Predicted MW is 25250.68 Da (+/- 10 Da by ESI-TOF). Observed MW is 25097.90 Da. M-100 due to loss of C-terminal arginine. -
氨基酸
19 to 238 -
额外的序列信息
N-terminal glycine.
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技术指标
Our Abpromise guarantee covers the use of ab280943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Mass Spectrometry
Sandwich ELISA
SDS-PAGE
HPLC
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
Buffer lyophilized from.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
常规信息
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别名
- B7 H
- B7 H1
- B7 homolog 1
see all -
功能
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
组织特异性
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
序列相似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane and Endomembrane system. - Information by UniProt
图片
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Functional Studies - Recombinant human PD-L1 protein (ab280943)
Functional analysis of an280943.
Fully biologically active measured by its ability to inhibit anti-CD3 and anti-CD8 antibody induced T-cell expansion. ED50 for this effect is ≤500 ng/mL with a specific activity of 0.2 x 104 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3381126-2
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SDS-PAGE - Recombinant Human PD-L1 protein (ab280943)SDS-PAGE analysis of ab280943.
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Sandwich ELISA - Recombinant human PD-L1 protein (Active) (ab280943)Sandwich ELISA - Recombinant human PD-L1 protein standard curve.
Background subtracted standard curve using Human/Monkey PD-L1 [28-8] Antibody Pair - BSA and Azide free (ab253485) and Recombinant human PD-L1 protein (Active) (ab280943) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.
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Mass Spectrometry - Recombinant Human PD-L1 protein (ab280943)Mass determination by ESI-TOF.
Predicted MW is 25250.68 Da (+/- 10 Da by ESI-TOF). Observed MW is 25097.90 Da. M-100 due to loss of C-terminal arginine.
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HPLC - Recombinant Human PD-L1 protein (ab280943)HPLC analysis of ab280943.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab280943 尚未被引用在任何文献中。
