重组人PCSK9 (mutated D374T)蛋白(Active) (Biotin) (ab271652)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 85% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, Avi tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
-
产品名称
重组人PCSK9 (mutated D374T)蛋白(Active) (Biotin)
参阅全部 PCSK9 蛋白酶 -
生物活性
Reaction was performed using a PCSK9(D374T)-LDLR TR-FRET Assay Kit. Briefly, 5 ng of europium-labeled LDLR were mixed with varying amounts of PCSK9(D374T) and dye labeled acceptor in PCSK9 TR-FRET assay buffer and incubated at RT for 2 hr. Donor emission was read at 620 nm followed by acceptor emission at 665 nm.
-
纯度
>= 85 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
序列
QEDEDGDYEELVLALRSEEDGLAEAPEHGTTATFHRCAKDPWRLPGTYVV VLKEETHLSQSERTARRLQAQAARRGYLTKILHVFHGLLPGFLVKMSGDL LELALKLPHVDYIEEDSSVFAQSIPWNLERITPPRYRADEYQPPDGGSLV EVYLLDTSIQSDHREIEGRVMVTDFENVPEEDGTRFHRQASKCDSHGTHL AGVVSGRDAGVAKGASMRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPV GPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAAGNFRDDACLYSPASA PEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSDCSTCFV SQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFP EDQRVLTPNLVAALPPSTHGAGWQLFCRTVWSAHSGPTRMATAVARCAPD EELLSCSSFSRSGKRRGERMEAQGGKLVCRAHNAFGGEGVYAIARCCLLP QANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLR PRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVTVACEEGW TLTGCSALPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSEGAVTAVAICCR SRHLAQASQELQ -
预测分子量
74 kDa -
氨基酸
31 to 692 -
修饰
mutated D374T -
标签
His tag C-Terminus , Avi tag C-Terminus -
额外的序列信息
Signal peptide is removed.
-
-
偶联物
Biotin
相关产品
-
Related Products
技术指标
Our Abpromise guarantee covers the use of ab271652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Functional Studies
SDS-PAGE
-
形式
Liquid -
补充说明
Enzymatically biotin-labeled using Avi-tag™ technology
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- Convertase subtilisin/kexin type 9 preproprotein
- FH3
- HCHOLA3
see all -
功能
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. -
组织特异性
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. -
疾病相关
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant. -
序列相似性
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain. -
翻译后修饰
The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. -
细胞定位
Secreted. - Information by UniProt
图片
-
Functional Studies - Recombinant human PCSK9 (mutated D374T) protein (Active) (Biotin) (ab271652)Specific activity of ab271652.
-
SDS-PAGE - Recombinant human PCSK9 (mutated D374T) protein (Active) (Biotin) (ab271652)SDS-PAGE analysis of 2.2 µg ab271652.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab271652 尚未被引用在任何文献中。