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AB229508

重组人PCK1/PEPC蛋白(Active)

Recombinant human PCK1/PEPC protein (Active)

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Recombinant human PCK1/PEPC protein (Active) is a Human Full Length protein, in the 2 to 622 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

查看别名

PEPCK1, PCK1, PEPCK-C, Serine-protein kinase PCK1

2 Images
Functional Studies - Recombinant human PCK1/PEPC protein (Active) (AB229508)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PCK1/PEPC protein (Active) (AB229508)

Activity of ab229508 was measured by a multi-steps assay. In this assay, PCK1/PEPC converts phosphoenolpyruvate into oxalacetate, then coupling with series of enzymatic reactions to generate pyruvate, which in turn reacts with a probe and developer to generate color (OD570 nm). Legend on the right indicated the amount of ab229508.

SDS-PAGE - Recombinant human PCK1/PEPC protein (Active) (AB229508)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PCK1/PEPC protein (Active) (AB229508)

4-20% SDS-PAGE analysis of ab229508 under reducing conditions and stained with Coomassie Blue.

Lane 1 : 0.5 μg; Lane 2 : 1 μg; Lane 3 : 1.5 μg.

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

Specific activity >100 mU/mg.

One unit is the amount of enzyme that transfers a phosphate group from PEP to GDP and generates 1.0 μmol of pyruvate per minute at pH 7.5 at 37oC

访问

P35558

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

Constituents: 5% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 5% Tris HCl, 3% Sodium chloride, 0.5% Magnesium chloride, 0.01% Manganese chloride

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

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Proteins & Peptides

AB101468

Recombinant Human CTNNBIP1/ICAT protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human CTNNBIP1/ICAT protein (His tag N-Terminus) (AB101468)

  • 0
  • 0
Proteins & Peptides

AB63175

Recombinant Human beta Catenin protein (GST tag N-Terminus)

Sandwich ELISA - Recombinant Human beta Catenin protein (Tagged) (AB63175)

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  • 0
Proteins & Peptides

AB285758

Recombinant Human GSK3 alpha fusion protein

SDS-PAGE - Recombinant Human GSK3 alpha fusion protein (AB285758)

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  • 0
Primary Antibodies

AB133603

Anti-PCK1/PEPC antibody [EPR6938]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCK1/PEPC antibody [EPR6938] (AB133603)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

This product is manufactured by BioVision, an Abcam company and was previously called P1229 Phosphoenolpyruvate Carboxykinase (PCK1), Human Recombinant. P1229-10 is the same size as the 10 μg size of ab229508.
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序列信息

[{"linker":null,"sequence":"PPQLQNGLNLSAKVVQGSLDSLPQAVREFLENNAELCQPDHIHICDGSEEENGRLLGQMEEEGILRRLKKYDNCWLALTDPRDVARIESKTVIVTQEQRDTVPIPKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLSKIGIELTDSPYVVASMRIMTRMGTPVLEAVGDGEFVKCLHSVGCPLPLQKPLVNNWPCNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRMASRLAKEEGWLAEHMLILGITNPEGEKKYLAAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWMKFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLASGVTITSWKNKEWSSEDGEPCAHPNSRFCTPASQCPIIDAAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTKLTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREILALKQRISQM","proteinLength":"Full Length","predictedMolecularWeight":"71.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":622,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P35558","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Lyophilized
运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein PCK1 also known as phosphoenolpyruvate carboxykinase PEPC or PEPCK plays an important role in gluconeogenesis by converting oxaloacetate to phosphoenolpyruvate (PEP). It has a molecular mass of around 69 kDa. PCK1 is primarily expressed in the liver kidney and adipose tissues. Through its enzymatic activity PCK1 helps to regulate blood glucose levels allowing for the production of glucose from non-carbohydrate sources.
Biological function summary

PCK1 is essential for maintaining glucose homeostasis in the body. Although not part of a larger protein complex its function directly impacts the tricarboxylic acid (TCA) cycle by providing substrates for glucose synthesis. By orchestrating the conversion of oxaloacetate to PEP PCK1 contributes significantly to reducing dependency on dietary carbohydrates particularly during fasting or intensive physical activity.

Pathways

PCK1 integrates critical metabolic processes. It mainly influences the gluconeogenesis and glycolysis pathways. In the gluconeogenesis pathway PCK1 coordinates with enzymes such as glucose-6-phosphatase to facilitate the generation of glucose from lactate and amino acids. Additionally its role in glycolysis intersects with enzymes like pyruvate kinase managing energy production and consumption.

PCK1 links to conditions such as diabetes mellitus and metabolic syndrome. Altered expression or function of PCK1 can lead to disturbances in glucose metabolism. In diabetes dysregulation of PCK1 expression can contribute to hyperglycemia. Additionally PCK1 and its interaction with insulin signaling pathways is critical as insulin resistance is an important feature of metabolic syndrome. Understanding these connections offers potential avenues for therapeutic intervention in metabolic diseases.
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常规信息

功能

Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis (PubMed : 24863970, PubMed : 26971250, PubMed : 28216384, PubMed : 30193097). Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle (PubMed : 24863970, PubMed : 26971250, PubMed : 28216384, PubMed : 30193097). At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed : 30193097). At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate (PubMed : 30193097). Acts as a regulator of formation and maintenance of memory CD8(+) T-cells : up-regulated in these cells, where it generates phosphoenolpyruvate, via gluconeogenesis (By similarity). The resultant phosphoenolpyruvate flows to glycogen and pentose phosphate pathway, which is essential for memory CD8(+) T-cells homeostasis (By similarity). In addition to the phosphoenolpyruvate carboxykinase activity, also acts as a protein kinase when phosphorylated at Ser-90 : phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as donor (PubMed : 32322062). The protein kinase activity regulates lipogenesis : upon phosphorylation at Ser-90, translocates to the endoplasmic reticulum and catalyzes phosphorylation of INSIG proteins (INSIG1 and INSIG2), thereby disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins (SREBF1/SREBP1 or SREBF2/SREBP2) and subsequent transcription of downstream lipogenesis-related genes (PubMed : 32322062).

序列相似性

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

翻译后修饰

Acetylated. Lysine acetylation by p300/EP300 is increased on high glucose conditions (PubMed:20167786, PubMed:21726808, PubMed:30193097). Lysine acetylation promotes ubiquitination by UBR5 (PubMed:21726808). Acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2. Deacetylation of Lys-91 is carried out by SIRT1 and depends on PCK1 phosphorylation levels (PubMed:30193097).. Phosphorylated in a GSK3B-mediated pathway; phosphorylation affects the efficiency of SIRT1-mediated deacetylation, and regulates PCK1 ubiquitination and degradation (PubMed:30193097). Phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes the protein kinase activity: phosphorylated PCK1 translocates to the endoplasmic reticulum, where it phosphorylates INSIG1 and INSIG2 (PubMed:32322062).. Ubiquitination by UBR5 leads to proteasomal degradation.

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产品实验方案

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靶点信息

Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis (PubMed : 24863970, PubMed : 26971250, PubMed : 28216384, PubMed : 30193097). Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle (PubMed : 24863970, PubMed : 26971250, PubMed : 28216384, PubMed : 30193097). At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed : 30193097). At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate (PubMed : 30193097). Acts as a regulator of formation and maintenance of memory CD8(+) T-cells : up-regulated in these cells, where it generates phosphoenolpyruvate, via gluconeogenesis (By similarity). The resultant phosphoenolpyruvate flows to glycogen and pentose phosphate pathway, which is essential for memory CD8(+) T-cells homeostasis (By similarity). In addition to the phosphoenolpyruvate carboxykinase activity, also acts as a protein kinase when phosphorylated at Ser-90 : phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as donor (PubMed : 32322062). The protein kinase activity regulates lipogenesis : upon phosphorylation at Ser-90, translocates to the endoplasmic reticulum and catalyzes phosphorylation of INSIG proteins (INSIG1 and INSIG2), thereby disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins (SREBF1/SREBP1 or SREBF2/SREBP2) and subsequent transcription of downstream lipogenesis-related genes (PubMed : 32322062).
See full target information PCK1
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