重组人PC4 (mutated S13 + S15 + S17 + S19)蛋白(ab81957)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, Functional Studies, GSA
描述
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产品名称
重组人PC4 (mutated S13 + S15 + S17 + S19)蛋白
参阅全部 PC4 蛋白酶 -
生物活性
1 unit equals 1 nanogram of purified protein. -
纯度
> 95 % SDS-PAGE.
ab81957 is purified by conventional chromatography. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
修饰
mutated S
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技术指标
Our Abpromise guarantee covers the use of ab81957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
Gel Supershift Assays
EMSA
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形式
Liquid -
补充说明
Wild-type PC4, but not the kinase inhibitory-deficient mutant of PC4 (serine mutations), represses specific transcription in vivo.
1 unit equals 1 nanogram of purified protein. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.9
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)
常规信息
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别名
- Activated RNA polymerase II transcriptional coactivator p15
- Interferon related developmental regulator 1
- MGC102747
see all -
功能
General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA). -
序列相似性
Belongs to the transcriptional coactivator PC4 family. -
翻译后修饰
Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region. -
细胞定位
Nucleus. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab81957 尚未被引用在任何文献中。