JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB123196

重组人 PBK/SPK 蛋白 (His tag N-Terminus)

Recombinant Human PBK/SPK protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(1 Publication)

Recombinant Human PBK/SPK protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 322 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

查看别名

TOPK, PBK, Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, T-LAK cell-originated protein kinase, CT84, SPK

1 Images
SDS-PAGE - Recombinant Human PBK/SPK protein (His tag N-Terminus) (AB123196)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PBK/SPK protein (His tag N-Terminus) (AB123196)

15% SDS-PAGE showing ab123196 (3μg).
(Molecular size on SDS-PAGE will appear higher).

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

Mass Spec, SDS-PAGE

applications

生物活性

No

访问

Q96KB5

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

This product was previously labelled as PBK
style
left-column

序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMEGISNFKTPSKLSEKKKSVLCSTPTINIPASPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPICNDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGSLCLAMEYGGEKSLNDLIEERYKASQDPFPAAIILKVALNMARGLKYLHQEKKLLHGDIKSSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTEPWKPKEAVEENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPPINMEELDESYQKVIELFSVCTNEDPKDRPSAAHIVEALETDV","proteinLength":"Full Length","predictedMolecularWeight":"38.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":322,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96KB5","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PBK/SPK also known as PDZ-binding kinase or Cdc2-related kinase is a serine/threonine protein kinase with an approximate molecular weight of 47 kDa. This protein is involved in cell division playing an important role in mitotic processes. PBK/SPK is expressed in various tissues including significant levels in testis and placenta often in proliferating cell types. It also exhibits higher expression in cancerous tissues suggesting a role in tumorigenesis.
Biological function summary

PBK/SPK facilitates cell cycle progression and cellular proliferation. This protein is not typically part of larger complexes but it partners with other molecules during cell division to ensure proper mitotic spindle formation and chromosome segregation. Its expression levels increase during the G2/M phase of the cell cycle indicating its involvement in cell cycle regulation. In cancer it often shows overexpression contributing to unregulated proliferation of cancer cells.

Pathways

PBK/SPK is associated with critical cell division pathways such as the MAPK signaling pathway. This involvement impacts cellular responses to growth signals and stress. PBK can phosphorylate and interact with proteins like p38 MAPK which further modulates signal transduction related to cellular growth and survival. Through these pathways PBK/SPK helps manage the balance between cell division and apoptosis particularly under stress conditions.

PBK/SPK has strong correlations with cancer especially glioblastoma and cervical cancer. Overexpression of PBK often links to poor prognosis in these conditions. In cancerous settings PBK interacts with proteins such as p53 and c-Myc influencing tumor progression and resistance to therapy. PBK can modulate oncogenic pathways providing a potential target for therapeutic intervention in tumor proliferation and survival.
style
left-column

特殊说明

形式

Liquid

附加说明

ab123196 is purified using conventional chromatography techniques.

style
left-column

常规信息

功能

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.

序列相似性

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

翻译后修饰

Phosphorylated; in a cell-cycle dependent manner at mitosis.

style
left-column

产品实验方案

style
left-column

靶点信息

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
See full target information PBK
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 38:1136-1150 PubMed30237440

2018

A reciprocal feedback between the PDZ binding kinase and androgen receptor drives prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Y Warren,Charlie E Massie,Kate Watt,Katarina Luko,Folake Orafidiya,Luke A Selth,Hisham Mohammed,Brinder S Chohan,Suraj Menon,Ajoeb Baridi,Wanfeng Zhao,Carles Escriu,Thanakorn Pungsrinont,Clive D'Santos,Xiaoping Yang,Chris Taylor,Arham Qureshi,Vincent R Zecchini,Greg L Shaw,Scott M Dehm,Ian G Mills,Jason S Carroll,Wayne D Tilley,Iain J McEwan,Aria Baniahmad,David E Neal,Mohammad Asim
View all publications
style
left-column
style
left-column
style
right-column

搭配使用产品

Primary Antibodies

AB236872

Anti-PBK/SPK antibody [EPR21983]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21983] (AB236872)

  • 0
  • 0
Proteins & Peptides

AB89692

Recombinant human TAK1 + TAB1 protein

Functional Studies - Recombinant human TAK1 + TAB1 protein (AB89692)

  • 0
  • 0
Proteins & Peptides

AB206806

Recombinant Human Glutaminyl cyclase protein (His tag C-Terminus)

SDS-PAGE - Recombinant Human Glutaminyl cyclase protein (His tag C-Terminus) (AB206806)

  • 0
  • 0
Proteins & Peptides

AB85295

Recombinant human SRPK1 protein

Functional Studies - Recombinant human SRPK1 protein (AB85295)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com