重组人 PARP2 蛋白 (GST tag N-Terminus)

常规信息

功能

Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair (PubMed : 10364231, PubMed : 25043379, PubMed : 27471034, PubMed : 30104678, PubMed : 32028527, PubMed : 32939087, PubMed : 34108479, PubMed : 34486521, PubMed : 34874266). Mediates glutamate, aspartate or serine ADP-ribosylation of proteins : the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (PubMed : 25043379, PubMed : 30104678, PubMed : 30321391). Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed : 32939087). Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1 (PubMed : 25043379). Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site (PubMed : 28190768, PubMed : 32028527, PubMed : 34108479, PubMed : 34486521, PubMed : 34874266). PARP2 initiates the repair of double-strand DNA breaks : recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks (PubMed : 10364231, PubMed : 32939087, PubMed : 34108479). HPF1 initiates serine ADP-ribosylation but restricts the polymerase activity of PARP2 in order to limit the length of poly-ADP-ribose chains (PubMed : 34732825, PubMed : 34795260). Specifically mediates formation of branched poly-ADP-ribosylation (PubMed : 30104678). Branched poly-ADP-ribose chains are specifically recognized by some factors, such as APLF (PubMed : 30104678). In addition to proteins, also able to ADP-ribosylate DNA : preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex (PubMed : 27471034, PubMed : 29361132).

序列相似性

Belongs to the ARTD/PARP family.

翻译后修饰

Auto poly-ADP-ribosylated on serine residues, leading to dissociation of the PARP2-HPF1 complex from chromatin (PubMed:32939087, PubMed:34108479). Poly-ADP-ribosylated by PARP1 (By similarity).. Acetylation reduces DNA binding and enzymatic activity.. Proteolytically cleaved by caspase-8 (CASP8) in response to apoptosis, leading to its inactivation.