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AB198628

重组人 PARP14 蛋白 (His tag N-Terminus + GST tag N-Terminus)

Recombinant human PARP14 protein (His tag N-Terminus + GST tag N-Terminus)

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Recombinant human PARP14 protein (His tag N-Terminus + GST tag N-Terminus) is a Human Fragment protein, in the 1470 to 1801 aa range, expressed in Baculovirus infected Sf9 cells, with >83%, suitable for SDS-PAGE, FuncS.

查看别名

BAL2, KIAA1268, PARP14, Protein mono-ADP-ribosyltransferase PARP14, ADP-ribosyltransferase diphtheria toxin-like 8, B aggressive lymphoma protein 2, Poly [ADP-ribose] polymerase 14, ARTD8, PARP-14

2 Images
Functional Studies - Recombinant human PARP14 protein (His tag N-Terminus + GST tag N-Terminus) (AB198628)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PARP14 protein (His tag N-Terminus + GST tag N-Terminus) (AB198628)

Specific activity of ab198628.

SDS-PAGE - Recombinant human PARP14 protein (His tag N-Terminus + GST tag N-Terminus) (AB198628)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PARP14 protein (His tag N-Terminus + GST tag N-Terminus) (AB198628)

4-20 % SDS-PAGE analysis of ab198628 with Coomassie staining.
Lane 1 : 1 μg ab198628
Lane 2 : Protein marker

关键信息

纯度

>83% SDS-PAGE

Affinity purified.

表达系统

Baculovirus infected Sf9 cells

标签

His tag N-Terminus GST tag N-Terminus

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form. Enzyme reaction is conducted using a PARP14 Assay Kit at room temperature for 1 hour.

访问

Q460N5

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Preservative: 1.28% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Primary Antibodies

AB302710

Anti-PARP14 antibody [EPR26299-91]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-PARP14 antibody [EPR26299-91] (AB302710)

  • 5
  • 1
Cell Lines & Lysates

AB259032

Human PARP14 knockout A549 cell lysate

Sanger Sequencing - Human PARP14 knockout A549 cell lysate (AB259032)

  • 0
  • 0
Sample Prep & Detection Kits

AB102890

HRP Conjugation Kit - Lightning-Link®

Conjugation - HRP Conjugation Kit - Lightning-Link® (AB102890)

  • 4
  • 3
Sample Prep & Detection Kits

AB269823

Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link®

Immunocytochemistry - Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (AB269823)

  • 5
  • 2
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"MHHHHHHGGGSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLVDFLSKLPEMLKMFKDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDPENLYFQGGGEFKEYQELNELQKKLNNISLDHKRPLIKVLGISRDVMQARDEIEAMIKRVRLAKEQESRADCISEFIEWQYNDNNTSHCFNKMTNLKLEDARREKKKTVDVKINHRHYTVNLNTYTATDTKGHSLSVQRLTKSKVDIPAHWSDKQQNFCVVELLPSDPEYNTVASKFNQTCSHFRIEKIERIQNPDLWNSYQAKKKTMDAKNGQTMNEKQLFHGTDAGSVPHVNRNGFNRSYAGKNAVAYGKGTYFAVNANYSANDTYSRPDANGRKHVYVRVLTGIYTHGNHSLIVPPSKNPQNPTDLYDTVTDNVHHPSLFVAFYDYQAYPEYLITFRK","proteinLength":"Fragment","predictedMolecularWeight":"66.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":1801,"aminoAcidStart":1470,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q460N5","tags":[{"tag":"His","terminus":"N-Terminus"},{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PARP14 also known as ADP-ribosyltransferase diphtheria toxin-like 8 (ARTD8) is a member of the poly(ADP-ribose) polymerase (PARP) family. It has a molecular mass approximately 180 kDa and is mainly found in the nucleus though it also localizes to the cytoplasm. This enzyme involves mono-ADP-ribosylation a process attaching ADP-ribose to target proteins which affects protein function and stability. It is expressed in various tissues with significant presence in immune cells.
Biological function summary

PARP14 participates in cell signaling and immune response regulation. It does not directly form part of a specific protein complex but interacts with several proteins influencing transcription and immune cell differentiation. PARP14 modulates the function of STAT6 a transcription factor vital for IL-4 induced gene expression in Th2 cell differentiation. This modulation plays a significant role in maintaining immune homeostasis and facilitating cellular communication during an immune response.

Pathways

PARP14 plays an integral role in the JAK-STAT and NF-kB signaling pathways. In the JAK-STAT pathway PARP14 modifies STAT6 activity which affects cytokine signaling and immune responses. Through the NF-kB pathway PARP14 can influence cell survival and inflammation by acting in combination with other PARP family members like PARP1 which shares a role in ADP-ribosylation signaling. These interactions ensure proper regulation of cellular stress responses and transcriptional activities.

PARP14 associations connect to cancer and inflammatory diseases. Its dysregulation can lead to altered immune responses which relate to cancers such as lymphoma. Additionally PARP14 influences inflammatory processes linked to disorders like asthma. Within these diseases abnormal PARP14 activity sometimes involves cross-talk with proteins like STAT6 in cancer pointing to its role in tumor-promoting inflammation and cell proliferation.
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常规信息

功能

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins (PubMed : 16061477, PubMed : 18851833, PubMed : 25043379, PubMed : 27796300). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed : 25043379). Has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', thus decreasing STAT1 phosphorylation which negatively regulates pro-inflammatory cytokine production in macrophages in response to IFNG stimulation (PubMed : 27796300). However, the role of ADP-ribosylation in the prevention of STAT1 phosphorylation has been called into question and it has been suggested that the inhibition of phosphorylation may be the result of sumoylation of STAT1 'Lys-703' (PubMed : 29858569). Mono-ADP-ribosylates STAT6; enhancing STAT6-dependent transcription (PubMed : 27796300). In macrophages, positively regulates MRC1 expression in response to IL4 stimulation by promoting STAT6 phosphorylation (PubMed : 27796300). Mono-ADP-ribosylates PARP9 (PubMed : 27796300).

序列相似性

Belongs to the ARTD/PARP family.

翻译后修饰

Auto-ADP-ribosylated.

亚细胞定位

Nucleus

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产品实验方案

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靶点信息

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins (PubMed : 16061477, PubMed : 18851833, PubMed : 25043379, PubMed : 27796300). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed : 25043379). Has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', thus decreasing STAT1 phosphorylation which negatively regulates pro-inflammatory cytokine production in macrophages in response to IFNG stimulation (PubMed : 27796300). However, the role of ADP-ribosylation in the prevention of STAT1 phosphorylation has been called into question and it has been suggested that the inhibition of phosphorylation may be the result of sumoylation of STAT1 'Lys-703' (PubMed : 29858569). Mono-ADP-ribosylates STAT6; enhancing STAT6-dependent transcription (PubMed : 27796300). In macrophages, positively regulates MRC1 expression in response to IL4 stimulation by promoting STAT6 phosphorylation (PubMed : 27796300). Mono-ADP-ribosylates PARP9 (PubMed : 27796300).
See full target information PARP14
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