AB51234

重组人 Parathyroid Hormone 蛋白 (Tag Free)

Name: 重组人Parathyroid Hormone蛋白 (ab51234)| Abcam中文官网
Brand: Abcam Limited
SKU: AB51234
Price: 3097 CNY
Availability: InStock

Recombinant Human Parathyroid Hormone protein (Tag Free)

Be the first to review this product! Submit a review

(1 Publication)

Recombinant Human Parathyroid Hormone protein (Tag Free) is a Human Full Length protein, in the 32 to 115 aa range, expressed in Escherichia coli, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, WB, ELISA.

查看别名

Parathyroid hormone, PTH, Parathormone, Parathyrin

3 Images

Sandwich ELISA - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

sELISA

Unknown

Sandwich ELISA - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

Standard curve for Parathyroid Hormone (Analyte : ab51234); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [A1/70] to Parathyroid Hormone (ab14493) at 0.2μg/ml and Detector Antibody Rabbit polyclonal to Parathyroid Hormone (ab53040) at 0.5μg/ml.

Unknown

Western blot - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

All lanes:

Western blot - Anti-Parathyroid Hormone antibody (<a href='/products/primary-antibodies/parathyroid-hormone-antibody-ab40630'>ab40630</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Parathyroid Hormone protein (Tag Free) (ab51234) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 13 kDa

true

Exposure time: 4min

SDS-PAGE - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

ab51234 on 15%SDS-PAGE

关键信息

纯度

>90% SDS-PAGE

内毒素水平

< 1 EU/µg

表达系统

Escherichia coli

应用

SDS-PAGE, WB, ELISA

applications

生物活性

访问

P01270

不含动物源

不含载体蛋白

种属

Human

存储溶液

pH: 7.4 Constituents: PBS, 0.0584% EDTA

storage-buffer

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab51234 can be used as a WB positive control in conjunction with <a href='/products/primary-antibodies/parathyroid-hormone-antibody-ab40630'>ab40630</a>." }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [A1/70] to Parathyroid Hormone (<a href='/products/primary-antibodies/parathyroid-hormone-antibody-a1-70-ab14493'>ab14493</a>) at 0.2μg/ml and Detector Antibody Rabbit polyclonal to Parathyroid Hormone (<a href='/products/unavailable/parathyroid-hormone-antibody-ab53040'>ab53040</a>) at 0.5μg/ml." } } }

style

left-column

style

left-column

序列信息

[{"sequence":"MSVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNFVALGAPLAPRDAGSQRPRKKEDNVLVESHEKSLGEADKADVNVLTKAKSQ","proteinLength":"Full Length","predictedMolecularWeight":"9.55 kDa","actualMolecularWeight":null,"aminoAcidEnd":115,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P01270","tags":[]}]

style

left-column

性能和储存信息

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

False

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Parathyroid hormone (PTH) also known as the parathyrin is a hormone protein with a molecular mass of about 9.4 kDa. It is synthesized and secreted by the chief cells of the parathyroid glands. PTH plays an important role in regulating calcium levels in the blood. Its molecular action involves binding to PTH receptors which are mainly located in bones and kidneys to increase calcium concentration by promoting its release from bones and reabsorption in the kidneys. This hormone is also present in the bloodstream as a parathyroid hormone peptide.

Biological function summary

PTH regulates calcium and phosphate metabolism in the body. It does not function as part of a larger complex but acts independently to control these important minerals. The hormone affects osteoclast activity in bones enhancing calcium and phosphate release. Additionally in the kidneys it reduces phosphate reabsorption while increasing calcium reabsorption. This balance maintained by PTH is essential for bone health and metabolic functions often associated with parathyroid hormone-related proteins in its activity.

Pathways

PTH is integral to the calcium signaling pathway and the renal phosphate reabsorption pathway. PTH works in concert with vitamin D to enhance intestinal calcium absorption. Calcitriol the active form of vitamin D depends on PTH for its synthesis in the kidneys linking the two in calcium homeostasis. Through these pathways PTH also interacts with the calcitonin hormone to moderate calcium levels creating a balance between mineral deposition and resorption in bones.

PTH is closely associated with hyperparathyroidism and hypoparathyroidism known as pan hypoparathyroidism when complete underactivity of the parathyroid glands occurs. In hyperparathyroidism an overproduction of PTH leads to high blood calcium levels and associated symptoms. Conversely hypoparathyroidism results in low calcium levels due to insufficient PTH secretion. Both conditions affect calcium metabolism and relate to proteins like calcium-sensing receptor and PTH-related peptide highlighting how critical PTH's function is in endocrine and metabolic health.

style

left-column

特殊说明

形式

Liquid

附加说明

Human Parathyroid Hormone(amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques.

style

left-column

常规信息

功能

Parathyroid hormone elevates calcium level by dissolving the salts in bone and preventing their renal excretion (PubMed : 11604398, PubMed : 35932760). Acts by binding to its receptor, PTH1R, activating G protein-coupled receptor signaling (PubMed : 18375760, PubMed : 35932760). Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells (PubMed : 21076856).

序列相似性

Belongs to the parathyroid hormone family.

style

left-column

产品实验方案

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

See full target information Parathyroid hormone

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Microbial cell factories 23:273 PubMed39390566

2024

Secretion of the human parathyroid hormone through a microcin type I secretion system in Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Flórez,Juan Marizcurrena,Magela Laviña,María F Azpiroz

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组人 Parathyroid Hormone 蛋白 (Tag Free)

Recombinant Human Parathyroid Hormone protein (Tag Free)

Sandwich ELISA - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

Western blot - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

SDS-PAGE - Recombinant Human Parathyroid Hormone protein (Tag Free) (AB51234)

关键信息

纯度

内毒素水平

表达系统

标签

应用

生物活性

访问

不含动物源

不含载体蛋白

种属

存储溶液