重组人PAK5蛋白(ab60754)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人PAK5蛋白 -
纯度
> 75 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab204849 (AKT1 peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- EC 2.7.11.1
- KIAA1264
- MGC26232
see all -
功能
The activated kinase acts on a variety of targets. -
组织特异性
Predominantly expressed in brain. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated when activated by CDC42/p21. - Information by UniProt
图片
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Functional Studies - Recombinant human PAK5 protein (ab60754)The specific activity of PAK5 (ab60754) was determined to be 14 nmol/min/mg as per activity assay protocol and was equivalent to 60 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human PAK5 protein (ab60754)SDS PAGE analysis of ab60754 -
Functional Studies - Recombinant human PAK5 protein (ab60754)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PAK5 protein (ab60754)ab60754 on SDS-PAGE, MW ~130kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab60754 被引用在 1 文献中.
- Pan YJ et al. Alternative splicing of HSPA12A pre-RNA by SRSF11 contributes to metastasis potential of colorectal cancer. Clin Transl Med 12:e1113 (2022). PubMed: 36394206