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AB60754

重组人PAK5蛋白

Recombinant human PAK5 protein

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(1 Publication)

Recombinant human PAK5 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, FuncS.

查看别名

KIAA1264, PAK7, PAK5, Serine/threonine-protein kinase PAK 5, p21-activated kinase 5, p21-activated kinase 7, PAK-5, PAK-7

4 Images
Functional Studies - Recombinant human PAK5 protein (AB60754)
  • FuncS

Unknown

Functional Studies - Recombinant human PAK5 protein (AB60754)

Sample Kinase Activity Plot.

Functional Studies - Recombinant human PAK5 protein (AB60754)
  • FuncS

Unknown

Functional Studies - Recombinant human PAK5 protein (AB60754)

The specific activity of PAK5 (ab60754) was determined to be 14 nmol/min/mg as per activity assay protocol and was equivalent to 60 nmol/min/mg as per radiometric assay

SDS-PAGE - Recombinant human PAK5 protein (AB60754)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PAK5 protein (AB60754)

ab60754 on SDS-PAGE, MW ~130kDa.

SDS-PAGE - Recombinant human PAK5 protein (AB60754)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PAK5 protein (AB60754)

SDS PAGE analysis of ab60754

关键信息

纯度

>75% Densitometry

表达系统

Baculovirus infected Sf9 cells

标签

GST tag N-Terminus

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

Active

访问

Q9P286

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

ab204849 (AKT1 peptide) can be utilized as a substrate for assessing kinase activity
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序列信息

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9P286","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.

序列相似性

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

翻译后修饰

Autophosphorylated when activated by CDC42/p21.

亚细胞定位

Mitochondrion

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产品实验方案

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靶点信息

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.
See full target information PAK5
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 12:e1113 PubMed36394206

2022

Alternative splicing of HSPA12A pre-RNA by SRSF11 contributes to metastasis potential of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Jie Pan,Fu-Chun Huo,Meng-Jie Kang,Bo-Wen Liu,Meng-Di Wu,Dong-Sheng Pei
View all publications
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搭配使用产品

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB110069

Anti-PAK5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAK5 antibody (AB110069)

  • 0
  • 0
Secondary Antibodies

AB191866

VHH Single Domain Anti-Rabbit IgG Fc (HRP)

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VHH Single Domain Anti-Rabbit IgG Fc (HRP) (AB191866)

  • 5
  • 2
Proteins & Peptides

AB254309

Recombinant human Alpha-Synuclein protein filament

Sandwich ELISA - Recombinant human Alpha-Synuclein protein filament (AB254309)

  • 0
  • 0

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