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AB196406

重组人 PADI2 / PAD2 蛋白 (DDDDK tag C-Terminus)

Recombinant human PADI2 / PAD2 protein (DDDDK tag C-Terminus)

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Recombinant human PADI2 / PAD2 protein is a Human Full Length protein, in the 1 to 665 aa range, expressed in Baculovirus infected Sf9, with >=61% purity and suitable for SDS-PAGE and Functional studies.The predicted molecular weight of ab196406 protein is 77 kDa.

- Save time and ensure accurate results - use our recombinant Human Peptidyl arginine deiminase 2 (PAD2/ PADI2) protein as a control
- Optimal protein bioactivity and stability

查看别名

KIAA0994, PAD2, PDI2, PADI2, Protein-arginine deiminase type-2, PAD-H19, Peptidylarginine deiminase II, Protein-arginine deiminase type II

2 Images
Functional Studies - Recombinant human PADI2 / PAD2 protein (DDDDK tag C-Terminus) (AB196406)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PADI2 / PAD2 protein (DDDDK tag C-Terminus) (AB196406)

Example of specific activity of ab196406

SDS-PAGE - Recombinant human PADI2 / PAD2 protein (DDDDK tag C-Terminus) (AB196406)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PADI2 / PAD2 protein (DDDDK tag C-Terminus) (AB196406)

4-20% SDS-PAGE analysis of ab196406 at 2.6 μg stained with Coomassie Blue (lane 1). Lane 2 is a protein marker.

关键信息

纯度

>61% SDS-PAGE

表达系统

Baculovirus infected Sf9 cells

标签

DDDDK tag C-Terminus

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

≥19 pmole/min/μg: Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl2, and 2 mM DTT) containing ab196406 and 10 mM BAEE as a substrate for 30 min at 37°C.

访问

Q9Y2J8

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.64% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Ensure the validity of your result using our bioactive recombinant human PADI2 / PAD2 as positive control in SDS-PAGE.


Check out our protein gel staining guide for SDS-PAGE here
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序列信息

[{"sequence":"MLRERTVRLQYGSRVEAVYVLGTYLWTDVYSAAPAGAQTFSLKHSEHVWVEVVRDGEAEEVATNGKQRWLLSPSTTLRVTMSQASTEASSDKVTVNYYDEEGSIPIDQAGLFLTAIEISLDVDADRDGVVEKNNPKKASWTWGPEGQGAILLVNCDRETPWLPKEDCRDEKVYSKEDLKDMSQMILRTKGPDRLPAGYEIVLYISMSDSDKVGVFYVENPFFGQRYIHILGRRKLYHVVKYTGGSAELLFFVEGLCFPDEGFSGLVSIHVSLLEYMAQDIPLTPIFTDTVIFRIAPWIMTPNILPPVSVFVCCMKDNYLFLKEVKNLVEKTNCELKVCFQYLNRGDRWIQDEIEFGYIEAPHKGFPVVLDSPRDGNLKDFPVKELLGPDFGYVTREPLFESVTSLDSFGNLEVSPPVTVNGKTYPLGRILIGSSFPLSGGRRMTKVVRDFLKAQQVQAPVELYSDWLTVGHVDEFMSFVPIPGTKKFLLLMASTSACYKLFREKQKDGHGEAIMFKGLGGMSSKRITINKILSNESLVQENLYFQRCLDWNRDILKKELGLTEQDIIDLPALFKMDEDHRARAFFPNMVNMIVLDKDLGIPKPFGPQVEEECCLEMHVRGLLEPLGLECTFIDDISAYHKFLGEVHCGTNVRRKPFTFKWWHMVP","proteinLength":"Full Length","predictedMolecularWeight":"77 kDa","actualMolecularWeight":null,"aminoAcidEnd":665,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9Y2J8","tags":[{"tag":"DDDDK","terminus":"C-Terminus"}]}]
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性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PADI2 also known as PAD2 or PADdeux is a protein that performs post-translational modification by converting arginine residues into citrulline residues in proteins through a process known as citrullination. This protein has an approximate mass of 75 kDa. PADI2 is expressed in many tissues but shows higher levels in the brain skeletal muscle and mammary gland. Within these tissues PADI2 plays a role in regulating various physiological processes by altering protein function through citrullination.
Biological function summary

PADI2 contributes significantly to the regulation of gene expression protein function and signal transduction. PADI2 does not exist as part of a larger protein complex yet it interacts with other proteins to regulate cellular processes. In particular it modifies histones which impacts chromatin structure and gene expression playing a role in cell differentiation and immune response modulation. Its activity can affect various cellular outcomes by regulating the function and interaction of different protein substrates.

Pathways

PADI2 takes part in gene regulation and cell differentiation processes by modifying histones and impacting chromatin structure. It is integral in pathways related to inflammation and immune responses such as the NF-kB signaling pathway enhancing its biological role. PADI2 interacts with multiple proteins including histones and other signaling molecules reinforcing its critical role in these pathways.

PADI2 has been linked to rheumatoid arthritis and multiple sclerosis due to its role in immune modulation and inflammation. In rheumatoid arthritis aberrant citrullination by PADI2 contributes to the production of autoantibodies which are involved in the disease's pathogenesis. It also associates with abnormal neural processes in multiple sclerosis where citrullinated proteins potentially contribute to the demyelination process. In these conditions PADI2's function relates closely with other citrullinating proteins such as PADI4 highlighting the interconnectedness of these pathways in disease.
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特殊说明

形式

Liquid

附加说明

Affinity purified.

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常规信息

功能

Catalyzes the deimination of arginine residues of proteins.

序列相似性

Belongs to the protein arginine deiminase family.

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产品实验方案

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靶点信息

Catalyzes the deimination of arginine residues of proteins.
See full target information PADI2
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搭配使用产品

Reagents, Controls & Accessories

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Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

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Proteins & Peptides

AB173032

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Primary Antibodies

AB16478

Anti-PADI2 / PAD2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PADI2 / PAD2 antibody (AB16478)

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Proteins & Peptides

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Recombinant human Hsp70 protein (Active)

Bioactive

SDS-PAGE - Recombinant human Hsp70 protein (Active) (AB78434)

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Proteins & Peptides

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Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein RBD (His tag)

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