JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB115719

重组人P4HB蛋白

Recombinant Human P4HB protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human P4HB protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB, FuncS.

查看别名

ERBA2L, PDI, PDIA1, PO4DB, P4HB, Protein disulfide-isomerase, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55

2 Images
Western blot - Recombinant Human P4HB protein (AB115719)
  • WB

Unknown

Western blot - Recombinant Human P4HB protein (AB115719)

All lanes:

Monoclonal mouse P4HB antibody

All lanes:

Western blot - Recombinant Human P4HB protein (ab115719) at 0.05 µg

false

SDS-PAGE - Recombinant Human P4HB protein (AB115719)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human P4HB protein (AB115719)

SDS-PAGE analysis
Lane 1 : MW marker
Lane 2 : ab115719 at 0.5 μg
Lane 3 : ab115719 at 1 μg
Lane 4 : ab115719 at 2 μg
Lane 5 : ab115719 at 5 μg

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

FuncS, SDS-PAGE, WB

applications

生物活性

No

访问

P07237

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

Constituents: 5% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.61% Tris, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

>200U/mg
style
left-column

序列信息

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"58 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P07237","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

性能和储存信息

运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

P4HB is a protein known as Protein Disulfide Isomerase (PDI). It plays a significant role in catalyzing the formation of disulfide bonds. This protein helps to fold newly synthesized proteins by shuffling disulfide bonds. It has a molecular mass of around 57 kDa. P4HB is mainly expressed in the endoplasmic reticulum of cells. While its alternative name is ERp59 P4HB is integral to the protein folding machinery inside cells.
Biological function summary

P4HB functions in protein folding and assembly. By acting as a chaperone it protects proteins from misfolding and aggregation. P4HB operates as part of a larger multi-protein complex that assists in maintaining protein structure under stress conditions in the cell. Its activity ensures protein stability and proper cellular function important for cell viability and health.

Pathways

P4HB plays a central role in the unfolded protein response (UPR) and oxidative protein folding pathway. It interacts with proteins such as calnexin and calreticulin through its involvement in these pathways. During oxidative protein folding P4HB introduces disulfide bonds into nascent proteins while removing incorrect ones ensuring efficient protein quality control.

P4HB alteration has connections to diseases like cancer and neurodegenerative disorders. Its overexpression and activity can promote tumor growth by aiding cancerous cell survival through protein homeostasis. Moreover P4HB's association with amyloid precursor protein impacts the progression of Alzheimer's disease. These connections make P4HB a relevant target for research into therapeutic interventions.
style
left-column

特殊说明

形式

Liquid

附加说明

ab115719 is purified by multi-step chromatography.

style
left-column

常规信息

功能

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations and following phosphorylation by FAM20C, functions as a chaperone that inhibits aggregation of misfolded proteins (PubMed : 32149426). At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed : 21670307).

序列相似性

Belongs to the protein disulfide isomerase family.

翻译后修饰

Phosphorylation of Ser-357 by FAM20C is induced by endoplasmic reticulum stress and results in a functional switch from oxidoreductase to molecular chaperone (PubMed:32149426). It also promotes interaction with ERN1 (PubMed:32149426).

style
left-column

产品实验方案

style
left-column

靶点信息

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations and following phosphorylation by FAM20C, functions as a chaperone that inhibits aggregation of misfolded proteins (PubMed : 32149426). At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed : 21670307).
See full target information P4HB
style
left-column
style
left-column
style
right-column

搭配使用产品

Proteins & Peptides

AB181966

Recombinant Dengue virus 2 NS1 protein

  • 0
  • 0
Primary Antibodies

AB137110

Anti-P4HB antibody [EPR9499]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-P4HB antibody [EPR9499] (AB137110)

  • 4
  • 6
Proteins & Peptides

AB95910

Recombinant E. coli Thioredoxin reductase protein

SDS-PAGE - Recombinant E. coli Thioredoxin reductase protein (AB95910)

  • 0
  • 0
Proteins & Peptides

AB206805

Recombinant human PDIA6 protein (His tag)

SDS-PAGE - Recombinant human PDIA6 protein (His tag) (AB206805)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com