重组人p38 delta/MAPK13蛋白(ab125643)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
重组人p38 delta/MAPK13蛋白
参阅全部 p38 delta/MAPK13 蛋白酶 -
生物活性
The specific activity of ab125643 was determined to be 120 nmol/min/mg as per activity assay protocol and equivalent to 151 nmol/min/mg as per radiometric assay
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纯度
> 90 % Densitometry.
Purity was determined to be >90% by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
71 kDa including tags -
氨基酸
1 to 365
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技术指标
Our Abpromise guarantee covers the use of ab125643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- MAP kinase 13
- MAP kinase p38 delta
- MAPK 13
see all -
功能
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. -
组织特异性
Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. - Information by UniProt
图片
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Functional Studies - Recombinant human p38 delta/MAPK13 protein (ab125643)The specific activity of MAPK 13 (ab125643) was determined to be 128 nmol/min/mg as per activity assay protocol and was equivalent to 161 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human p38 delta/MAPK13 protein (ab125643)SDS PAGE analysis of ab125643 -
SDS-PAGE - Recombinant human p38 delta/MAPK13 protein (ab125643)SDS PAGE analysis of ab125643 -
SDS-PAGE - Recombinant human p38 delta/MAPK13 protein (ab125643)SDS-PAGE analysis of ab125643. -
Functional Studies - Recombinant human p38 delta/MAPK13 protein (ab125643)The specific activity of ab125643 was determined to be 140 nmol/min/mg by Kinase Assay.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab125643 尚未被引用在任何文献中。