重组人OGT / O-Linked N-Acetylglucosamine Transferase蛋白

特殊说明

形式

Liquid

常规信息

功能

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc) (PubMed : 12150998, PubMed : 15361863, PubMed : 19451179, PubMed : 20018868, PubMed : 21240259, PubMed : 21285374, PubMed : 23103939, PubMed : 26237509, PubMed : 26369908, PubMed : 26678539, PubMed : 27713473, PubMed : 37541260, PubMed : 37962578). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, AMPK, ATG4B, CAPRIN1, EZH2, FNIP1, GSDMD, KRT7, LMNA, LMNB1, LMNB2, RPTOR, HOXA1, PFKL, KMT2E/MLL5, MAPT/TAU, TET2, RBL2, RET, NOD2 and HCFC1 (PubMed : 19451179, PubMed : 20200153, PubMed : 21285374, PubMed : 22923583, PubMed : 23353889, PubMed : 24474760, PubMed : 26237509, PubMed : 26369908, PubMed : 26678539, PubMed : 27527864, PubMed : 30699359, PubMed : 34074792, PubMed : 34667079, PubMed : 37541260, PubMed : 37962578). Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing (PubMed : 21285374). Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling (By similarity). Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity (PubMed : 22923583). Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B : recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3) (PubMed : 22121020, PubMed : 23353889). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed : 20018852). O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex (PubMed : 24474760). Stabilizes KMT2E/MLL5 by mediating its glycosylation, thereby preventing KMT2E/MLL5 ubiquitination (PubMed : 26678539). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver (By similarity). Stabilizes clock proteins BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation (By similarity). Promotes the CLOCK-BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2. O-glycosylates HCFC1 and regulates its proteolytic processing and transcriptional activity (PubMed : 21285374, PubMed : 28302723, PubMed : 28584052). Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1 (PubMed : 20200153). Regulates mitochondrial motility in neurons by mediating glycosylation of TRAK1 (By similarity). Promotes autophagy by mediating O-glycosylation of ATG4B (PubMed : 27527864). Acts as a regulator of mTORC1 signaling by mediating O-glycosylation of RPTOR and FNIP1 : O-GlcNAcylation of RPTOR in response to glucose sufficiency promotes activation of the mTORC1 complex (PubMed : 30699359, PubMed : 37541260).. Isoform 2. The mitochondrial isoform (mOGT) is cytotoxic and triggers apoptosis in several cell types including INS1, an insulinoma cell line.. Isoform 4. Has N-acetylglucosaminyltransferase activity : glycosylates proteins, such as HNRNPU, NEUROD1, NUP62 and PDCD6IP (PubMed : 31527085). Displays specific substrate selectivity compared to other isoforms (PubMed : 31527085).

序列相似性

Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily.

翻译后修饰

Ubiquitinated, leading to its proteasomal degradation.. Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity (By similarity). Phosphorylation at Thr-454 by AMPK promotes nuclear localization (PubMed:24563466).. Glycosylated via autocatalysis; O-GlcNAcylation at Ser-399 promotes nuclear localization.. Isoform 4. Glycosylated via autocatalysis; does not affect the enzyme activity but regulates substrate selectivity.

亚细胞定位

Nucleus