重组人OGT / O-Linked N-Acetylglucosamine Transferase蛋白(ab131881)
Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE, ELISA
描述
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产品名称
重组人OGT / O-Linked N-Acetylglucosamine Transferase蛋白 -
表达系统
Wheat germ -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHCMQLWRQ EPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYK ERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSAL QYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCV FNAQGEIWLAIHHFEKAVTLDPNFLDAYINLGNVLKEARIFDRAVAAYLR ALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAIELQPHFPDAYCNLA NALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIEEAVRLY RKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSN MGNTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIA SYRTALKLKPDFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKN RLPSVHPHHSMLYPLSHGFRKAIAERHGNLCLDKINVLHKPPYEHPKDLK LSDGRLRVGYVSSDFGNHPTSHLMQSIPGMHNPDKFEVFCYALSPDDGTN FRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIHILVNMNGYTKGARNEL FALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQYSEKLAYMP HTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPD VKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQITIN GFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQL YKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRI IFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGET LASRVAASQLTCLGCLELIAKNRQEYEDIAVKLGTDLEYLKKVRGKVWKQ RISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPVEVTESA -
预测分子量
143 kDa including tags -
氨基酸
1 to 1046 -
标签
GST tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab131881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
SDS-PAGE
ELISA
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
常规信息
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别名
- FLJ23071
- GlcNAc transferase
- HRNT1
see all -
功能
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. -
组织特异性
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. -
通路
Protein modification; protein glycosylation. -
序列相似性
Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats. -
结构域
The TPR repeat domain mediates recognition of protein substrates. -
翻译后修饰
Ubiquitinated, leading to its proteasomal degradation. -
细胞定位
Cytoplasm. Nucleus. Mostly in the nucleus. - Information by UniProt
图片
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SDS-PAGE - Recombinant Human OGT / O-Linked N-Acetylglucosamine Transferase protein (ab131881)12.5% SDS-PAGE stained with Coomassie Blue showing ab131881 at approximately 143.3 kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab131881 被引用在 1 文献中.
- Yu M et al. O-GlcNAcylation of ITGA5 facilitates the occurrence and development of colorectal cancer. Exp Cell Res 382:111464 (2019). PubMed: 31202709
