AB103059

重组人 NUDT16 蛋白 (His tag N-Terminus)

Name: 重组人NUDT16蛋白 (ab103059)| Abcam中文官网
Brand: Abcam Limited
SKU: AB103059
Price: 3872 CNY
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Recombinant Human NUDT16 protein (His tag N-Terminus)

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(2 Publications)

Recombinant Human NUDT16 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 195 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

查看别名

U8 snoRNA-decapping enzyme, IDP phosphatase, Inosine diphosphate phosphatase, Nucleoside diphosphate-linked moiety X motif 16, Nudix hydrolase 16, U8 snoRNA-binding protein H29K, m7GpppN-mRNA hydrolase, IDPase, Nudix motif 16, NUDT16

1 Images

SDS-PAGE - Recombinant Human NUDT16 protein (His tag N-Terminus) (AB103059)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NUDT16 protein (His tag N-Terminus) (AB103059)

15% SDS-PAGE (3 μg)

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

应用

Mass Spec, SDS-PAGE

applications

生物活性

访问

Q96DE0

不含动物源

不含载体蛋白

种属

Human

存储溶液

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAGARRLELGEALALGSGWRHACHALLYAPDPGMLFGRIPLRYAILMQMRFDGRLGFPGGFVDTQDRSLEDGLNRELREELGEAAAAFRVERTDYRSSHVGSGPRVVAHFYAKRLTLEELLAVEAGATRAKDHGLEVLGLVRVPLYTLRDGVGGLPTFLENSFIGSAREQLLEALQDLGLLQSGSISGLKIPAHH","proteinLength":"Full Length","predictedMolecularWeight":"23.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":195,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96DE0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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性能和储存信息

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

False

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NUDT16 also known as diphosphatase is an enzyme that belongs to the nudix hydrolase family. It has a molecular mass of approximately 19 kDa. This enzyme cleaves diphosphate groups from nucleoside diphosphate derivatives. NUDT16 is highly expressed in several tissues including liver and testis. Its activity is important for maintaining cellular nucleotide pool balance by removing non-canonical nucleotides that can potentially be harmful if incorporated into DNA or RNA.

Biological function summary

NUDT16 functions to regulate nucleotide metabolism. It catalyzes the hydrolysis of nucleoside diphosphates like diadenosine tetraphosphate into monophosphates. NUDT16 often operates with other proteins in multi-enzyme complexes where it plays a role in modulating the concentrations of nucleoside diphosphates within cells. This activity coordinates the synthesis and degradation of nucleotides influencing cellular energy status and coenzyme levels.

Pathways

With regards to pathways NUDT16 integrates into nucleotide catabolism and salvage pathways. These pathways facilitate the conversion and recycling of nucleotides ensuring a steady supply for nucleic acid synthesis. NUDT16 interacts within these pathways alongside other key enzymes such as IMP dehydrogenase and GMP synthetase highlighting its role in controlling guanine nucleotide levels. This involvement provides a direct link to DNA replication and repair mechanisms which depend on the accurate control of nucleotide pools.

NUDT16 has connections to cancer and autoimmune diseases. Abnormal activity or expression of NUDT16 may contribute to the accumulation of atypical nucleotides potentially leading to mutations and genomic instability that characterize several cancer types. In autoimmune contexts altered NUDT16 function can impact immune cell activation and response. It exhibits an intriguing relationship with proteins like SAMHD1 a known regulator of nucleotide pools involved in immune responses and cancer.

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特殊说明

形式

Liquid

附加说明

ab103059 was purified using conventional chromatography techniques.

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常规信息

功能

RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Also catalyzes the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Also acts as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms (PubMed : 20385596, PubMed : 26121039). The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP (PubMed : 20385596). Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions (PubMed : 20385596). Exhibits decapping activity towards NAD-capped RNAs and FAD-capped RNAs (PubMed : 32432673). Exhibits decapping activity towards dpCoA-capped RNAs in vitro (By similarity).

序列相似性

Belongs to the Nudix hydrolase family. NUDT16 subfamily.

亚细胞定位

Nucleus

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information NUDT16

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 52:801-815 PubMed38000390

2023

DELTEX E3 ligases ubiquitylate ADP-ribosyl modification on nucleic acids.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Zhu,Marcin J Suskiewicz,Chatrin Chatrin,Øyvind Strømland,Bryan W Dorsey,Vincent Aucagne,Dragana Ahel,Ivan Ahel

Science advances 8:eadd4253 PubMed36197986

2022

DELTEX E3 ligases ubiquitylate ADP-ribosyl modification on protein substrates.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Zhu,Marcin J Suskiewicz,Andrea Hloušek-Kasun,Hervé Meudal,Andreja Mikoč,Vincent Aucagne,Dragana Ahel,Ivan Ahel

View all publications

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重组人 NUDT16 蛋白 (His tag N-Terminus)

Recombinant Human NUDT16 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human NUDT16 protein (His tag N-Terminus) (AB103059)

关键信息

纯度

表达系统

标签

应用

生物活性

访问

不含动物源

不含载体蛋白

种属

存储溶液