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AB106873

重组人 NPL 蛋白 (His tag N-Terminus)

Recombinant Human NPL protein (His tag N-Terminus)

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Recombinant Human NPL protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 320 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

查看别名

C1orf13, NPL, N-acetylneuraminate lyase, NALase, N-acetylneuraminate pyruvate-lyase, N-acetylneuraminic acid aldolase, Sialate lyase, Sialate-pyruvate lyase, Sialic acid aldolase, Sialic acid lyase

1 Images
SDS-PAGE - Recombinant Human NPL protein (His tag N-Terminus) (AB106873)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human NPL protein (His tag N-Terminus) (AB106873)

15% SDS-PAGE analysis of 3μg ab106873.

关键信息

纯度

>95% SDS-PAGE

ab106873 is purified using conventional chromatography techniques.

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

Mass Spec, SDS-PAGE

applications

生物活性

No

访问

Q9BXD5

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

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Primary Antibodies

AB223521

Anti-NPL antibody

Immunocytochemistry/ Immunofluorescence - Anti-NPL antibody (AB223521)

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Proteins & Peptides

AB48737

Recombinant Human Retinoid X Receptor alpha/RXRA protein (Tag Free)

SDS-PAGE - Recombinant Human Retinoid X Receptor alpha/RXRA protein (Tag Free) (AB48737)

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  • 0
Primary Antibodies

AB195020

Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

RabMAb|Recombinant|KO Validated|Trial Size

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB195020)

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Primary Antibodies

AB244403

Anti-GNE antibody

Immunocytochemistry/ Immunofluorescence - Anti-GNE antibody (AB244403)

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  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMAFPKKKLQGLVAATITPMTENGEINFSVIGQYVDYLVKEQGVKNIFVNGTTGEGLSLSVSERRQVAEEWVTKGKDKLDQVIIHVGALSLKESQELAQHAAEIGADGIAVIAPFFLKPWTKDILINFLKEVAAAAPALPFYYYHIPALTGVKIRAEELLDGILDKIPTFQGLKFSDTDLLDFGQCVDQNRQQQFAFLFGVDEQLLSALVMGATGAVGSTYNYLGKKTNQMLEAFEQKDFSLALNYQFCIQRFINFVVKLGFGVSQTKAIMTLVSGIPMGPPRLPLQKASREFTDSAEAKLKSLDFLSFTDLKDGNLEAGS","proteinLength":"Full Length","predictedMolecularWeight":"37.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":320,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9BXD5","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The NPL protein also known as N-acetylneuraminate pyruvate lyase is an enzyme that catalyzes the reversible cleavage of N-acetylneuraminic acid (Neu5Ac) into pyruvate and N-acetylmannosamine. This protein weighs approximately 34 kDa and takes part in the sialic acid metabolism pathway. NPL is mainly expressed in the liver and kidney tissues in humans. It demonstrates a structural requirement for metal ions such as magnesium or manganese as co-factors to stabilize its active form.
Biological function summary

N-acetylneuraminate pyruvate lyase influences the recycling and regulation of sialic acids which are vital components of cell surface molecules involved in cellular recognition and signaling. This protein acts as part of a larger metabolic network involving sialic acid biosynthesis and degradation. It supports cellular functions by recycling sialic acid which gets added to glycoproteins and glycolipids contributing to cell surface architecture and function.

Pathways

The sialic acid metabolism involving the NPL protein connects to the broader glycosylation pathways impacting cell-cell communication and pathogen recognition. The NPL protein interacts with enzymes like sialyltransferases which transfer sialic acids to glycan chains and it maintains the rate of Neu5Ac production and consumption. It also relates to neuraminidases which cleave sialic acids from glycoconjugates ensuring dynamic metabolic fluxes in cells.

The function of NPL protein ties to the regulation of sialic acid levels impacting conditions such as sialuria and macrophage activity in sepsis. Sialuria is associated with excessive Neu5Ac accumulation due to malfunctioning NPL. Additionally altered sialic acid levels can affect immune responses in sepsis where NPL indirectly influences pathways involving other proteins such as sialoadhesins which bind to sialic acids on cell surfaces.
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常规信息

功能

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate (PubMed : 33895133). It prevents sialic acids from being recycled and returning to the cell surface (PubMed : 33895133). Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway (PubMed : 22692205, PubMed : 33895133). Although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded (Probable).

序列相似性

Belongs to the DapA family. NanA subfamily.

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产品实验方案

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靶点信息

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate (PubMed : 33895133). It prevents sialic acids from being recycled and returning to the cell surface (PubMed : 33895133). Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway (PubMed : 22692205, PubMed : 33895133). Although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded (Probable).
See full target information NPL
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