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AB281817

重组人Noggin蛋白(Active)

Recombinant human Noggin protein (Active)

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(1 Publication)

Recombinant human Noggin protein (Active) is a Human Full Length protein, in the 28 to 232 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

查看别名

Noggin, NOG

4 Images
Mass Spectrometry - Recombinant human Noggin protein (Active) (AB281817)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human Noggin protein (Active) (AB281817)

Mass determination by ESI-TOF.

Predicted MW is 23104.64 Da. (+/- 10 Da by ESI-TOF). Observes MW is 23106.53 Da. Additional masses at 23382.04 is due to residual O-glycans.

Functional Studies - Recombinant human Noggin protein (Active) (AB281817)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Noggin protein (Active) (AB281817)

Functional analysis of ab281817.

Fully biologically active determined by its ability to inhibit BMP-4 induced alkaline phosphatase activity in ATDC5 cells.

ED50 for this effect is ≤65.37 ng/ml, corresponding to a specific activity of 1.52 x 104 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : ​GR3387043-1

HPLC - Recombinant human Noggin protein (Active) (AB281817)
  • HPLC

Supplier Data

HPLC - Recombinant human Noggin protein (Active) (AB281817)

HPLC analysis of ab281817.

SDS-PAGE - Recombinant human Noggin protein (Active) (AB281817)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Noggin protein (Active) (AB281817)

SDS-PAGE analysis of ab281817.

关键信息

纯度

>95% SDS-PAGE

内毒素水平

<0.005 EU/µg

表达系统

HEK 293 cells

标签

Tag free

应用

FuncS, Mass Spec, HPLC, SDS-PAGE, Cell Culture

applications

生物活性

Yes

生物学活性

Fully biologically active determined by its ability to inhibit BMP-4 induced alkaline phosphatase activity in ATDC5 cells.

ED50 for this effect is ≤65.37 ng/ml, corresponding to a specific activity of 1.52 x 104 units/mg.

访问

Q13253

不含动物源

Yes

不含载体蛋白

No

种属

Human

复溶

Reconstitute in PBS

存储溶液

pH: 7.4 Constituents: 10% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"QHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC","proteinLength":"Full Length","predictedMolecularWeight":"23.1 kDa","actualMolecularWeight":"23.11 kDa","aminoAcidEnd":232,"aminoAcidStart":28,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q13253","tags":[]}]
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性能和储存信息

运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
Ambient
推荐的长期储存条件
Ambient
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Noggin also known as noggin protein is a secreted glycoprotein with a mass of approximately 26 kDa. It antagonizes bone morphogenetic proteins (BMPs) by directly binding them preventing their interaction with receptors. This binding inhibits the signaling pathway which affects cellular processes such as differentiation growth and apoptosis. Noggin expresses prominently in embryonic tissues and in tissues undergoing differentiation particularly in the neural tube notochord and developing articular cartilage. The expression pattern suggests its importance during critical phases of development and tissue patterning.
Biological function summary

Noggin functions as an essential regulator in embryonic development sculpting tissues by inhibiting BMP activity. It does not work alone but functions as part of a complex regulatory network involving other BMP antagonists. By inhibiting BMPs Noggin plays an important role in neural tube closure limb formation and somite development. Noggin's activities help modulate the balance between proliferation and differentiation in these contexts allowing for proper structural formation during embryogenesis.

Pathways

Noggin operates mainly within the TGF-beta/BMP signaling pathway serving as a critical modulator. Inhibiting BMP through Noggin affects downstream targets connecting it to other signaling proteins like SMADs which mediate transcriptional responses. This control enables Noggin to indirectly regulate Wnt signaling as well showing its impact on various developmental stages and processes. Its influence on these pathways indicates Noggin's importance in maintaining cellular homeostasis during growth and morphogenesis.

Noggin relates to conditions such as fibrodysplasia ossificans progressiva and cartilage-related abnormalities. Mutations or dysregulation of noggin protein can disrupt normal BMP signaling leading to aberrant skeletal development and calcification. This disruption often involves interactions with other proteins like BMP4 revealing Noggin's critical role in maintaining bone and cartilage integrity. Targeting noggin pathway components may provide therapeutic potential for addressing such conditions.
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特殊说明

形式

Lyophilized

附加说明

>=95% Purity by HPLC

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常规信息

功能

Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (PubMed : 21976273, PubMed : 26643732).

序列相似性

Belongs to the noggin family.

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产品实验方案

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靶点信息

Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (PubMed : 21976273, PubMed : 26643732).
See full target information NOG
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 23:594 PubMed37370018

2023

High glucose promotes the progression of colorectal cancer by activating the BMP4 signaling and inhibited by glucagon-like peptide-1 receptor agonist.

Applications

Unspecified application

Species

Unspecified reactive species

Bingwei Ma,Xingchun Wang,Hui Ren,Yingying Li,Haijiao Zhang,Muqing Yang,Jiyu Li
View all publications
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