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AB271638

重组人 NNMT 蛋白 (Active) (His tag C-Terminus)

Recombinant human NNMT protein (Active) (His tag C-Terminus)

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Recombinant human NNMT protein (Active) (His tag C-Terminus) is a Human Full Length protein, in the 1 to 264 aa range, expressed in HEK 293 cells, with >90%, suitable for SDS-PAGE, FuncS.

查看别名

Nicotinamide N-methyltransferase, NNMT

2 Images
Functional Studies - Recombinant human NNMT protein (Active) (His tag C-Terminus) (AB271638)
  • FuncS

Supplier Data

Functional Studies - Recombinant human NNMT protein (Active) (His tag C-Terminus) (AB271638)

Specifc activity of ab271638 was 57 pmol/min/μg.

SDS-PAGE - Recombinant human NNMT protein (Active) (His tag C-Terminus) (AB271638)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human NNMT protein (Active) (His tag C-Terminus) (AB271638)

SDS-PAGE analysis of ab271638.

关键信息

纯度

>90% SDS-PAGE

表达系统

HEK 293 cells

标签

His tag C-Terminus

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

Specific activity: 57 pmol/min/μg

Assay Conditions: 10 mM Tris-HCl, pH 7.4, 0.05% Tween 20, 1 mM DTT, 100 μM Quinoline, 100 μM SAM, 30 min, room temperature

访问

P40261

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Preservative: 0.99% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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搭配使用产品

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Primary Antibodies

AB119758

Anti-NNMT antibody [OTI3D8]

KO Validated

Western blot - Anti-NNMT antibody [OTI3D8] (AB119758)

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  • 3
Cell Lines & Lysates

AB258537

Human NNMT knockout HeLa cell lysate

Western blot - Human NNMT knockout HeLa cell lysate (AB258537)

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Proteins & Peptides

AB114288

Recombinant Human FUT4 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human FUT4 protein (GST tag N-Terminus) (AB114288)

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Proteins & Peptides

AB316388

Recombinant Human CD63 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human CD63 Protein Standard (His tag) (AB316388)

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  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL","proteinLength":"Full Length","predictedMolecularWeight":"30 kDa","actualMolecularWeight":null,"aminoAcidEnd":264,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P40261","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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性能和储存信息

形式
Liquid
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The NNMT protein also known as Nicotinamide N-methyltransferase acts by catalyzing the methylation of nicotinamide using S-adenosylmethionine as a methyl donor. This enzyme has a mass of around 29 kDa and is expressed in various tissues including liver adipose tissue and certain cancer cells. The NNMT gene provides instructions for creating the protein that plays significant roles in metabolic processes and cellular homeostasis.
Biological function summary

NNMT impacts cell energy balance and gene expression regulation by influencing the levels of nicotinamide and methionine metabolites. This enzyme does not typically form part of a complex acting more so as an individual regulator of methylation reactions affecting cellular metabolism. Researchers find interest in NNMT for its role in modulating cellular growth and proliferation forming a link to both normal cellular functions and pathological conditions like cancer.

Pathways

NNMT has a significance in NAD+ metabolism and methionine salvage pathways. These pathways are essential in maintaining redox homeostasis and proper methylation status in cells. NNMT through its enzymatic activity interacts with SAM (S-adenosyl methionine) closely relating its function to methylation processes that involve other proteins such as SIRTs (Sirtuins) and NAMPT (Nicotinamide phosphoribosyltransferase) within these metabolic pathways.

NNMT shows relevance in cancer and obesity-related complications. The protein's upregulation in cancer types like breast and colorectal cancers links it to tumor development and progression. In obesity NNMT's involvement in adipose tissue might contribute to metabolic dysregulation. The enzyme also interconnects with proteins like insulin receptors and adiponectin highlighting its potential role in metabolic diseases and making it an interesting target for therapeutic interventions.
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常规信息

功能

Catalyzes the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PubMed : 21823666, PubMed : 23455543, PubMed : 8182091). Plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3 (PubMed : 23455543, PubMed : 26571212, PubMed : 31043742). In a developmental context, contributes to low levels of the repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state (PubMed : 26571212). Acts as a metabolic regulator primarily on white adipose tissue energy expenditure as well as hepatic gluconeogenesis and cholesterol biosynthesis. In white adipocytes, regulates polyamine flux by consuming S-adenosyl-L-methionine which provides for propylamine group in polyamine biosynthesis, whereas by consuming nicotinamide controls NAD(+) levels through the salvage pathway (By similarity). Via its product N1-methylnicotinamide regulates protein acetylation in hepatocytes, by repressing the ubiquitination and increasing the stability of SIRT1 deacetylase (By similarity). Can also N-methylate other pyridines structurally related to nicotinamide and play a role in xenobiotic detoxification (PubMed : 30044909).

序列相似性

Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.

翻译后修饰

Deiminated by PADI1 and PADI2.

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产品实验方案

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靶点信息

Catalyzes the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PubMed : 21823666, PubMed : 23455543, PubMed : 8182091). Plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3 (PubMed : 23455543, PubMed : 26571212, PubMed : 31043742). In a developmental context, contributes to low levels of the repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state (PubMed : 26571212). Acts as a metabolic regulator primarily on white adipose tissue energy expenditure as well as hepatic gluconeogenesis and cholesterol biosynthesis. In white adipocytes, regulates polyamine flux by consuming S-adenosyl-L-methionine which provides for propylamine group in polyamine biosynthesis, whereas by consuming nicotinamide controls NAD(+) levels through the salvage pathway (By similarity). Via its product N1-methylnicotinamide regulates protein acetylation in hepatocytes, by repressing the ubiquitination and increasing the stability of SIRT1 deacetylase (By similarity). Can also N-methylate other pyridines structurally related to nicotinamide and play a role in xenobiotic detoxification (PubMed : 30044909).
See full target information Nicotinamide N-methyltransferase
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