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AB211318

重组人 NME3 蛋白 (His tag N-Terminus)

Recombinant human NME3 protein (His tag N-Terminus)

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Recombinant human NME3 protein (His tag N-Terminus) is a Human Fragment protein, in the 22 to 169 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS, Mass Spec.

查看别名

Nucleoside diphosphate kinase C, NDPK-C, DR-nm23, Nucleoside diphosphate kinase 3, nm23-H3, NDK3, NME3

1 Images
SDS-PAGE - Recombinant human NME3 protein (His tag N-Terminus) (AB211318)
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Supplier Data

SDS-PAGE - Recombinant human NME3 protein (His tag N-Terminus) (AB211318)

15% SDS-PAGE analysis of ab211318 (3μg).

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

标签

His tag N-Terminus

应用

SDS-PAGE, Mass Spec, FuncS

applications

生物活性

Yes

生物学活性

Specific activity is > 150 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25°C in a couple system with PK/LDH.

访问

Q13232

不含动物源

No

不含载体蛋白

No

种属

Human

存储溶液

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMERTFLAVKPDGVQRRLVGEIVRRFERKGFKLVALKLVQASEELLREHYAELRERPFYGRLVKYMASGPVVAMVWQGLDVVRTSRALIGATNPADAPPGTIRGDFCIEVGKNLIHGSDSVESARREIALWFRADELLCWEDSAGHWLYE","proteinLength":"Fragment","predictedMolecularWeight":"19.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":169,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q13232","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NME3 also known as non-metastatic cells protein 3 is a part of the nucleoside diphosphate kinase (NDPK) family. This protein has a molecular mass of approximately 17.3 kDa. Scientists have reported its expression in various tissues with particular abundance in lungs and brain. NME3 interacts with nucleotides participating in phosphate transfer reactions by converting nucleoside diphosphates into nucleoside triphosphates.
Biological function summary

Non-metastatic cells protein 3 plays a role in maintaining cellular nucleotide homeostasis. NME3 is a member of a larger NDPK complex working closely with other NDPK family members like NME1 and NME2. This complex contributes to DNA replication and repair processes. It also influences cell proliferation and differentiation by regulating the levels of GTP and other nucleotides important for these biological processes.

Pathways

There is significant involvement of non-metastatic cells protein 3 in cellular energy metabolism and signal transduction pathways. NME3 participates in the Ras signaling pathway where it interacts with Ras proteins regulating their activation state. Additionally NME3 functions within the Wnt signaling pathway contributing to cell cycle regulation and development.

Non-metastatic cells protein 3 associates with cancer where altered NME3 expression correlates with tumor progression and metastasis. Studies indicate that changes in NME3 can disrupt interactions with proteins like NME1 influencing cancer cell motility and invasion. NME3 also connects with neurodegenerative disorders with dysregulation possibly affecting cellular processes that protect against neuronal cell death.
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特殊说明

形式

Liquid

附加说明

ab211318 was purified using conventional chromatography.

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常规信息

功能

Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis (PubMed : 11277919, PubMed : 30587587, PubMed : 39337255). In vitro, can also use other phosphate donors such as UTP and GTP (PubMed : 30587587, PubMed : 39337255). Independently of its nucleoside diphosphate kinase activity, involved in mitochondrial membrane tethering, a prerequisite for fusion through direct membrane-binding and hexamerization (PubMed : 30587587, PubMed : 37584589). Involved in DNA repair of both single- and double-stranded breaks by associating with the ribonucleotide reductase (RNR) complex via interaction with the histone acetyltransferase KAT5, facilitating recruitment to DNA damage sites independently of its kinase activity (PubMed : 26945015). Inhibits granulocyte differentiation (PubMed : 7638209). May be required for ciliary function during renal development (By similarity).

序列相似性

Belongs to the NDK family.

翻译后修饰

Autophosphorylation is Mg(2+)-dependent.

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产品实验方案

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靶点信息

Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis (PubMed : 11277919, PubMed : 30587587, PubMed : 39337255). In vitro, can also use other phosphate donors such as UTP and GTP (PubMed : 30587587, PubMed : 39337255). Independently of its nucleoside diphosphate kinase activity, involved in mitochondrial membrane tethering, a prerequisite for fusion through direct membrane-binding and hexamerization (PubMed : 30587587, PubMed : 37584589). Involved in DNA repair of both single- and double-stranded breaks by associating with the ribonucleotide reductase (RNR) complex via interaction with the histone acetyltransferase KAT5, facilitating recruitment to DNA damage sites independently of its kinase activity (PubMed : 26945015). Inhibits granulocyte differentiation (PubMed : 7638209). May be required for ciliary function during renal development (By similarity).
See full target information Nucleoside diphosphate kinase 3
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